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首页> 外文期刊>Journal of Clinical Microbiology >Analysis of Vibrio parahaemolyticus soluble antigens by employing passive hemagglutination.
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Analysis of Vibrio parahaemolyticus soluble antigens by employing passive hemagglutination.

机译：通过使用被动血凝分析副溶血弧菌可溶性抗原。

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摘要

The passive hemagglutination assay was explored as a sensitive test of immunological reactivity of endotoxin and other antigens prepared from selected strains of Vibrio parahaemolyticus. The tannic acid procedure for passive hemagglutination, commonly used with protein antigens, was the only procedure yielding good results with V. parahaemolyticus protein extracts, endotoxins, and related preparations. These results were probably due to the presence of large amounts of protein in the V. parahaemolyticus endotoxins as determined by earlier work referenced in the text. Glucose and galactose as possible antigenic determinants in the endotoxin of a Vibrio strain were tested by inhibition tests. Cross-reactions were observed between endotoxin preparations, but were low in hemagglutination, suggesting reactions of common generic antigens. The ability of V. parahaemolyticus endotoxins to stimulate production of antibodies was determined.
机译：探索了被动血凝测定法作为对内毒素和从选定的副溶血弧菌菌株制备的其他抗原的免疫反应性的敏感测试。鞣制酸用于被动血凝的方法，通常与蛋白质抗原一起使用，是唯一用副溶血弧菌蛋白提取物，内毒素和相关制剂产生良好效果的方法。这些结果可能是由于副溶血性弧菌内毒素中存在大量蛋白质所致，这是通过本文中引用的较早工作确定的。通过抑制试验测试了弧菌菌株内毒素中葡萄糖和半乳糖可能的抗原决定簇。在内毒素制剂之间观察到交叉反应，但是血凝反应低，表明常见的通用抗原反应。确定了副溶血弧菌内毒素刺激抗体产生的能力。

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