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首页> 外文期刊>Journal of Clinical Microbiology >Solid-phase enzyme-linked immunosorbent assay for detection of hepatitis A-specific immunoglobulin M.
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Solid-phase enzyme-linked immunosorbent assay for detection of hepatitis A-specific immunoglobulin M.

机译：固相酶联免疫吸附法检测甲型肝炎特异性免疫球蛋白M

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摘要

A solid-phase enzyme linked immunosorbent assay was developed for the detection of immunoglobulin M antibody to hepatitis A virus. The system was capable of detecting hepatitis A-specific immunoglobulin M in a single dilution of serum and appears to be a reliable and rapid means of establishing a diagnosis of hepatitis A infection. Specific immunoglobulin M was only detected in patients with serologically confirmed hepatitis A and not in patients with other forms of hepatitis, chronic liver disease, or autoimmune disease. In patients with hepatitis A, specific immunoglobulin M was usually detectable for 6 weeks after the onset of dark urine, and the longest period for which it was present in any patient was 115 days. This enzyme-linked immunosorbent assay is rapid, simple to perform, and does not require complicated equipment. Provided adequate supplies of purified reagents can be obtained, this enzyme-linked immunosorbent assay procedure is likely to simplify hepatitis A serology, because the same antibody-coated plates can be utilized to detect hepatitis A virus, anti-hepatitis A virus, and hepatitis A-specific immunoglobulin M.
机译：开发了一种固相酶联免疫吸附测定法，用于检测针对甲型肝炎病毒的免疫球蛋白M抗体。该系统能够在单次稀释的血清中检测到甲型肝炎特异性免疫球蛋白M，似乎是建立甲型肝炎感染诊断的可靠且快速的手段。仅在血清学确诊的甲型肝炎患者中检测到特异性免疫球蛋白M，而在其他形式的肝炎，慢性肝病或自身免疫性疾病患者中未检测到。在患有甲型肝炎的患者中，通常在深色尿液发作后的6周内可检测到特异性免疫球蛋白M，并且在任何患者中存在的最长时间为115天。这种酶联免疫吸附测定快速，易于执行，并且不需要复杂的设备。如果能够获得足够的纯化试剂供应，该酶联免疫吸附测定程序可能会简化甲型肝炎的血清学检测，因为相同的抗体包被的板可用于检测甲型肝炎病毒，抗甲型肝炎病毒和甲型肝炎特异性免疫球蛋白M

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