Sensitivity, precision, and accuracy of the Y1 adrenal cell enterotoxin assay.

J H Tenney; T F Smith; J A Washington

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Sensitivity, precision, and accuracy of the Y1 adrenal cell enterotoxin assay.

机译：Y1肾上腺细胞肠毒素测定的灵敏度，精密度和准确性。

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The Y1 adrenal cell assay for heat-labile enterotoxin (LT) was found to be relatively insensitive for filtrates of toxigenic Escherichia coli H10407. When observations were made blindly and subjected to rigorous controls, reliable detection of LT occured only at filtrate dilutions from 1:4 to 1:10. Detection of LT was unreliable when E. coli H10407 was mixed with another enteric organism. Overall, 7.1% of observers' reports from a single assay were imprecise, and 2.6% would have resulted in errors in detection of LT; however, triplicate assays prevented false positive reports of LT detection. Routine testing for LT production in the clinical diagnostic laboratory awaits a simpler, more sensitive, and more direct method of testing stool specimens.

机译：发现热不稳定肠毒素（LT）的Y1肾上腺细胞测定法对产毒性大肠杆菌H10407的滤液相对不敏感。当盲目观察并进行严格控制时，仅当滤液稀释度为1：4至1:10时，可靠地检测到LT。当大肠杆菌H10407与另一种肠溶菌混合时，LT的检测是不可靠的。总体而言，一次测定中7.1％的观察者报告不准确，而2.6％的结果将导致LT检测错误;但是，一式三份的测定可防止LT检测的假阳性报告。在临床诊断实验室中对LT生产进行例行测试正在等待一种更简单，更灵敏，更直接的测试粪便标本的方法。

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《Journal of Clinical Microbiology》 |1979年第2期|共3页
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J H Tenney; T F Smith; J A Washington;
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中图分类医学微生物学（病原细菌学、病原微生物学）;
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Sensitivity, precision, and accuracy of the Y1 adrenal cell enterotoxin assay.

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