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首页> 外文期刊>Journal of Clinical Microbiology >Diagnosis of recent rubella virus infection by demonstration of specific immunoglobulin M antibodies: comparison of solid-phase reverse immunosorbent test with sucrose density gradient centrifugation.
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Diagnosis of recent rubella virus infection by demonstration of specific immunoglobulin M antibodies: comparison of solid-phase reverse immunosorbent test with sucrose density gradient centrifugation.

机译：通过展示特定的免疫球蛋白M抗体来诊断最近的风疹病毒感染：固相逆向免疫吸附试验与蔗糖密度梯度离心法的比较。

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摘要

A solid-phase reverse immunosorbent test (SPRIST) based on the addition of an excess of rubella virus hemagglutinin was evaluated for the demonstration of rubella-specific immunoglobulin M (IgM), and the results were compared with those of the density gradient centrifugation technique. In a retrospective study in which 157 sera were tested, the two techniques yielded identical results (55 IgM-positive and 102 IgM-negative samples). In a prospective study, 592 sere were examined; 8 IgM-positive results by SPRIST corresponded to a recent rubella infection or vaccination. Neither rheumatoid factor nor heterophil antibody seemed to interfere with the results of SPRIST. This test would be a useful and rapid routine technique for demonstration of the presence of virus-specific IgM in serum samples, particularly for viruses with a hemagglutinin. Except for anti-human IgM, no more reagents are needed than for widely used hemagglutination inhibition procedures.
机译：评估了基于添加过量风疹病毒血凝素的固相逆向免疫吸附试验（SPRIST），以证明风疹特异性免疫球蛋白M（IgM），并将结果与​​密度梯度离心技术进行了比较。在一项回顾性研究中，测试了157个血清，这两种技术产生了相同的结果（55个IgM阳性和102个IgM阴性样品）。在一项前瞻性研究中，检查了592处硒。 SPRIST的8 IgM阳性结果与最近的风疹感染或疫苗接种相对应。类风湿因子和异源抗体似乎都不会干扰SPRIST的结果。该测试对于证明血清样品中病毒特异性IgM的存在将是一种有用且快速的常规技术，特别是对于具有血凝素的病毒而言。除了抗人IgM，不需要比广泛使用的血凝抑制程序更多的试剂。

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