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首页> 外文期刊>Journal of Clinical Microbiology >Detection and characterization of mouse monoclonal antibodies to epidemic typhus rickettsiae.
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Detection and characterization of mouse monoclonal antibodies to epidemic typhus rickettsiae.

机译：流行性伤寒立克次体的小鼠单克隆抗体的检测和表征。

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A solid-phase immunofluorometric assay was used to detect mouse monoclonal antibodies to epidemic typhus rickettsiae, Rickettsia prowazekii (the immunizing antigen), and to murine typhus rickettsiae, Rickettsia typhi, a related antigen. Of the 649 hybridoma cultures obtained, 628 contained antibodies either to R. prowazekii or to both R. prowazekii and R. typhi. A total of 72 cultures were cloned by limiting dilution and yielded 137 antibody-producing clones. Of these, 104 produced antibodies specific for R. prowazekii, 22 produced antibodies that reacted with R. prowazekii and R. typhi, and 11 produced antibodies that reacted with R. prowazekii, R. typhi, and R. canada. The immunoglobulin isotypes of the mouse monoclonal antibodies produced were identified by a related indirect immunofluorometric assay technique with fluorescein isothiocyanate-conjugated antisera specific for each isotype. Antibodies were also evaluated by indirect fluorescent antibody tests, and antibodies from selected clones were found to neutralize rickettsial toxic activity in mice.
机译：固相免疫荧光测定法用于检测针对流行性立克次氏病立克次体立克次体（免疫抗原）和针对鼠类立克次氏病立克次体相关性抗原的小鼠单克隆抗体。在获得的649种杂交瘤培养物中，有628种所含的抗体既有针对R. prowazekii的抗体，也包括针对R. prowazekii和R. typhi的抗体。通过有限稀释克隆总共72个培养物，得到137个产生抗体的克隆。在这些抗体中，有104种产生了对R. prowazekii的抗体，22种产生了与R. prowazekii和R. typhi反应的抗体，11种产生了与R. prowazekii，R. typhi和R.canada反应的抗体。通过相关的间接免疫荧光测定技术，使用对每种同种型特异的荧光素异硫氰酸酯缀合的抗血清，鉴定产生的小鼠单克隆抗体的免疫球蛋白同种型。还通过间接荧光抗体测试评估了抗体，发现来自选定克隆的抗体可中和小鼠的立克次氏体毒性活性。

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