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首页> 外文期刊>Journal of Clinical Microbiology >Examination of the morphology of bacteria adhering to peritoneal dialysis catheters by scanning and transmission electron microscopy.
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Examination of the morphology of bacteria adhering to peritoneal dialysis catheters by scanning and transmission electron microscopy.

机译：通过扫描和透射电子显微镜检查附着在腹膜透析导管上的细菌的形态。

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We examined Tenckhoff peritoneal catheters by scanning and transmission electron microscopy to study the morphology of bacterial adherence. Two catheters were removed from uninfected patients, three from patients with exit site infections, four from patients with peritonitis, and one from a patient with both exit site infection and peritonitis. Infecting organisms included three of Staphylococcus aureus and one each of Enterobacter sp., Staphylococcus epidermidis, Achromobacter xylosoxidans, Serratia sp., Klebsiella sp., and Candida albicans. Considerable morphological variation in adherence to the peritoneal dialysis apparatus occurred. No inflammatory cells were ever seen in association with infected cuffs, only two of the five patients with peritonitis had inflammatory cells associated with their catheters. In both instances, these cells tended to occur in clumps and demonstrated no flattening when in contact with the surface. Colonization of the catheter was uneven--bacteria tended to occur in clusters. Extensive matrix formation was evident in several instances, and condensation of this matrix onto the bacteria during the dehydration process rendered clumps of bacterial cells amorphous at times. Bacteria were adherent to the subcutaneous cuff in those patients with exit site infections. Gram-negative bacteria attached to individual dacron fibers of the cuff, often several layers deep. Gram-positive bacteria tended to adhere in clusters.
机译：我们通过扫描和透射电子显微镜检查了Tenckhoff腹膜导管，以研究细菌粘附的形态。从未感染的患者中取出两根导管，从出口部位感染的患者中取出三根导管，从腹膜炎患者中取出四根，并从出口部位感染和腹膜炎患者中取出一根。感染生物包括三个金黄色葡萄球菌和每个肠杆菌，表皮葡萄球菌，木氧化无色杆菌，沙雷氏菌，克雷伯菌和白色念珠菌。遵守腹膜透析仪的形态发生了很大变化。从未见过与感染的袖带相关的炎性细胞，在五名腹膜炎患者中，只有两个患有与导管相关的炎性细胞。在这两种情况下，这些细胞都倾向于成团出现，并且与表面接触时不会展平。导管的集落不均匀-细菌倾向于成簇出现。在几种情况下，明显形成了广泛的基质，并且在脱水过程中该基质在细菌上的凝结有时会使细菌细胞团变成无定形的。在患有出口部位感染的患者中，细菌粘附在皮下袖带上。革兰氏阴性细菌附着在袖带的单个涤纶纤维上，通常深达几层。革兰氏阳性细菌倾向于成簇粘附。

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