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首页> 外文期刊>Journal of Clinical Microbiology >Serological classification of Achromobacter xylosoxidans.
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Serological classification of Achromobacter xylosoxidans.

机译：木氧化无色杆菌的血清学分类。

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Adult rabbits were immunized with nine Achromobacter xylosoxidans strains by intravenous injection of Formalin-killed organisms. Antisera thus obtained were reciprocally titrated with the nine A. xylosoxidans strains, and seven sera were defined as serologically distinct. Three of nine antisera possessed one common antibody while also each having their own specific antibody. Ninety-five strains of A. xylosoxidans were examined for serotyping by a microtiter agglutination test with the nine antisera, and the strains were divided into seven serogroups. The distribution of the 95 strains among the serogroups was as follows: serogroup A, 15 strains; B, 17 strains; C, 43 strains; D, 2 strains; E, 4 strains; G, 8 strains; F, 2 strains. Four strains were not agglutinated with any of the antisera. Serogroups A, B, and F had the ability to reduce nitrate to nitrogen gas and to grow in heart infusion broth at 41 degrees C, although there were some exceptions. In contrast, most strains of serogroups C, D, and E could not produce nitrogen gas from nitrate, although they did produce nitrite. The strains of serogroup C could not grow at 41 degrees C, whereas those of serogroups D and E could. Thus, we concluded that serogroups A, B, and F referred to biotype IIIb described by Tatum, and serogroups C, D, and E referred to biotype IIIa. The serotyping of A. xylosoxidans may be useful for the analysis of nosocomial infections caused by these organisms.
机译：通过静脉内注射福尔马林杀死的生物，用九种木糖氧化无色杆菌菌株对成年兔进行免疫。如此获得的抗血清用九种木糖氧化木霉菌株相互滴定，并且七个血清被定义为血清学上不同的。九种抗血清中的三种拥有一种共同抗体，而每种也都有自己的特异性抗体。通过用九种抗血清的微量滴定凝集试验，检查了九十五种木糖氧化农杆菌的血清分型，并将这些菌株分为七个血清组。 95个菌株在血清群中的分布如下：血清群A，15个菌株;和。 B，17株; C，43株; D，2株; E，4株; G，8株; F，2株。四个菌株未与任何抗血清凝集。血清群A，B和F具有将硝酸盐还原为氮气并在41°C的心脏浸液中生长的能力，尽管有一些例外。相反，大多数血清群C，D和E的菌株虽然确实产生亚硝酸盐，但它们不能从硝酸盐中产生氮气。血清群C的菌株不能在41℃下生长，而血清群D和E的菌株可以。因此，我们得出结论，血清群A，B和F指的是Tatum描述的生物型IIIb，血清群C，D和E指的是生物型IIIa。木糖氧化过氧化物酶的血清分型可用于分析由这些生物体引起的医院感染。

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