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首页> 外文期刊>Journal of Clinical Microbiology >Rapid and reliable method for production of a specific Paracoccidioides brasiliensis immunodiffusion test antigen.
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Rapid and reliable method for production of a specific Paracoccidioides brasiliensis immunodiffusion test antigen.

机译：快速而可靠的方法来生产特定的巴西拟南芥免疫扩散测试抗原。

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Previously published methods to produce Paracoccidioides brasiliensis antigens for serological tests have yielded antigens of inconsistent quality and have involved the use of special semisynthetic media and growth periods of 1 to 3 months to yield suitable reagents. A simple procedure that uses commercially available potato glucose agar and either SABHI broth (Difco Laboratories) or Trypticase soy broth (BBL Microbiology Systems) inoculated with the mycelial form of P. brasiliensis consistently yielded high-titer antigens in 2 weeks or less. This new method permits the almost exclusive production of an antigen identical to the specific E antigen described by Yarzabal (Yarzabal et al., Sabouradia 14:275-280, 1976) and the apparently equivalent specific antigen 1 described by Restrepo and Moncada (A. Restrepo and L. H. Moncada, Appl. Microbiol. 28:138-144, 1974). In the immunodiffusion test, the rapidly produced antigen demonstrated a sensitivity of 90% by detecting antibody in sera from 103 of 114 proven cases of paracoccidioidomycosis. The specificity of this antigen was 100% because none of 139 sera from patients with heterologous mycotic diseases demonstrated diagnostic precipitins against the P. brasiliensis antigen. In the complement fixation tests, the rapidly produced antigen was not as suitable as the one prepared by the method of Restrepo-Moreno and Schneidau (A. Restrepo-Moreno and J. D. Schneidau, Jr., J. Bacteriol. 93:1741-1748, 1967).
机译：先前发表的生产用于血清学测试的巴西副球菌抗原的方法产生的抗原质量不一致，并且涉及使用特殊的半合成培养基，生长时间为1-3个月，以产生合适的试剂。使用市售马铃薯葡萄糖琼脂和SABHI肉汤（Difco Laboratories）或胰酶解大豆肉汤（BBL Microbiology Systems）接种了巴西利亚毕赤酵母菌丝体形式的简单方法，可在2周或更短时间内持续产生高滴度抗原。该新方法允许几乎独家生产与Yarzabal（Yarzabal等人，Sabouradia 14：275-280，1976）描述的特异性E抗原相同的抗原以及Restrepo和Moncada（A. Restrepo和LH Moncada，Appl.Microbiol.28：138-144，1974）。在免疫扩散试验中，通过检测114例经证实的副球孢子菌病病例中的103例血清中的抗体，快速产生的抗原显示出90％的敏感性。该抗原的特异性为100％，因为来自异源性真菌病患者的139份血清均未显示针对巴西假单胞菌抗原的诊断性沉淀素。在补体固定测试中，快速产生的抗原不如通过Restrepo-Moreno和Schneidau（A. Restrepo-Moreno和JD Schneidau，Jr.，J. Bacteriol。93：1741-1748， 1967）。

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