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首页> 外文期刊>Journal of Clinical Microbiology >Identification of Achromobacter species by cellular fatty acids and by production of keto acids.
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Identification of Achromobacter species by cellular fatty acids and by production of keto acids.

机译:通过细胞脂肪酸和酮酸的生产来鉴定无色杆菌物种。

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The cellular fatty acid composition and metabolic products of 12 reference strains of Achromobacter sp. and A. xylosoxidans were determined by gas-liquid chromatography (GLC). Results showed that the two Achromobacter groups are strikingly different and can be readily distinguished on the basis of cellular fatty acids and the short-chain acids produced by Achromobacter sp. The major cellular fatty acids of Achromobacter sp. were octadecenoic (18:1) and a 19-carbon cyclopropanoic (19:0 delta) acid, whereas hexadecanoic (16:0) and a 17-carbon cyclopropanoic (17:0 delta) acid were principal components of the lipids of A. xylosoxidans. Hydroxy acids were not found in strains of Achromobacter sp. but comprised approximately 20% of the cellular fatty acids of A. xylosoxidans. In addition, Achromobacter sp. produced relatively large amounts of 2-ketoisocaproic acid, which was detected in only trace amounts from strains of A. xylosoxidans. The data show that GLC tests provide additional criteria for differentiating groups which are very closely related when evaluated with conventional tests. The GLC tests can be readily adapted in the clinical laboratory because they are rapid, highly reproducible, relatively inexpensive, and simple to perform.
机译:12种无色杆菌参考菌株的细胞脂肪酸组成和代谢产物。通过气相色谱法(GLC)测定木糖氧化酶和木糖氧化过氧化物酶。结果表明,两个无色杆菌基团截然不同,并且可以根据细胞脂肪酸和由无色杆菌属物种产生的短链酸容易地区分。无色杆菌属的主要细胞脂肪酸。它们是十八烯酸(18:1)和19碳环丙酸(19:0δ)酸,而十六烷酸(16:0)和17碳环丙酸(17:0δ)酸是A脂质的主要成分。木糖氧化酶。在无色杆菌属菌株中未发现羟基酸。但包含约20%的木糖氧化过氧化物酶细胞脂肪酸。另外,无色杆菌属。产生相对大量的2-酮异己酸,仅从木糖农杆菌菌株中检​​出痕量。数据表明,GLC测试为区分组别提供了其他标准,这些标准在与常规测试进行评估时非常相关。 GLC测试可以快速,高度可重复,相对便宜且易于执行,因此可以在临床实验室中轻松进行调整。

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