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首页> 外文期刊>Journal of Clinical Microbiology >Improved version of the exoantigen test for identification of Coccidioides immitis and Histoplasma capsulatum cultures.
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Improved version of the exoantigen test for identification of Coccidioides immitis and Histoplasma capsulatum cultures.

机译：改良版的exoantigen测试，用于鉴定球虫球菌性炎和荚膜组织胞浆菌培养物。

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摘要

A rapid and simple method for extracting specific cell-free antigens of Coccidioides immitis and Histoplasma capsulatum from agar slant cultures was developed. The extracts were analyzed in immunodiffusion tests for the presence of C. immitis or H. capsulatum specific antigens. These extracts were compared with culture filtrates of brain heart infusion broth subcultures in tests with 32 isolates of C. immitis and H. capsulatum and 13 other fungi which might be morphologically confused with them. The studies showed that the slant extracts were as useful as the culture filtrates and allowed more rapid identification of C. immitis and H. capsulatum. In every case, when an identification was made by conventional morphological methods, the immunological test yielded correlating results. The immunological tests were positive for two isolates that were converted to their yeast forms only after 4 months of study by conventional tests. The new procedure permits the identification of C. immitis and H. capsulatum 2 days after receipt of a pure mycelial-form culture. The test is recommended for the presumptive identification of C. immitis and H. capsulatum cultures.
机译：开发了一种快速简便的方法，可从琼脂斜面培养物中提取球虫球菌和荚膜组织胞浆的特定无细胞抗原。在免疫扩散测试中分析提取物是否存在C. immitis或荚膜H.荚膜特异性抗原。将这些提取物与脑心浸液肉汤亚培养物的培养物滤液进行了比较，测试了32种分离的C. immitis和H.荚膜菌和13种其他真菌，在形态上可能与它们混淆。研究表明，倾斜的提取物与培养滤液一样有用，可以更快速地鉴定C. immitis和荚膜H.。在每种情况下，当通过常规形态学方法进行鉴定时，免疫学检测均产生相关结果。两种分离株的免疫学试验均为阳性，这些分离株仅在常规试验研究四个月后才转变为酵母形式。新程序允许在收到纯菌丝体形式培养物后2天鉴定出C.炎性肠炎和荚膜H.荚膜炎。推荐该测试用于鉴定假单胞菌和荚膜包涵体培养物。

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