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首页> 外文期刊>Journal of Clinical Microbiology >Biotin-avidin-amplified enzyme immunoassay for detection of herpes simplex virus antigen in clinical specimens.
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Biotin-avidin-amplified enzyme immunoassay for detection of herpes simplex virus antigen in clinical specimens.

机译：生物素-亲和素扩增酶免疫法检测临床标本中的单纯疱疹病毒抗原。

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A biotin-avidin-amplified enzyme-linked immunosorbent assay (B-A ELISA) has been developed to detect herpes simplex virus type 1 (HSV-1) and HSV-2 antigens in clinical specimens. The test was designed as a solid-phase, double-antibody, sandwich assay in which plates were coated with a polyclonal rabbit immunoglobulin G anti-HSV reagent, and the sandwich antibody was a biotin-labeled mouse immunoglobulin M monoclonal antibody that reacts with a common antigen associated with HSV-1 and HSV-2. The test can be completed in 4 h if antibody-coated plates are available. The detection limit of the B-A ELISA, determined by titration of virus stocks, was found to be approximately 90 PFU or 6 X 10(3) physical particles of either HSV-1 or HSV-2 per 50 microliter of virus stock. The following results were obtained in a study in which swabs were taken from a variety of lesions and assayed for infectivity in tissue culture and by B-A ELISA. Of 421 suspected HSV lesions tested, 69 were positive by both tests and 159 were negative by both tests. A total of 122 were positive by B-A ELISA but negative for infectivity. Seventy-one were negative by B-A ELISA but contained infectious virus. The HSV specificity of the assay was substantiated by partial blocking of reactivity with rabbit immunoglobulin G anti-HSV and by the absence of reactivity with a nonspecific biotin-labeled mouse immunoglobulin M monoclonal antibody.
机译：已开发出一种生物素-亲和素扩增的酶联免疫吸附测定法（B-A ELISA），以检测临床标本中的1型单纯疱疹病毒（HSV-1）和HSV-2抗原。该测试被设计为固相双抗体夹心测定法，其中板被多克隆兔免疫球蛋白G抗HSV试剂包被，并且夹心抗体是生物素标记的小鼠免疫球蛋白M单克隆抗体，可与抗体反应。与HSV-1和HSV-2相关的常见抗原。如果有抗体包被的板，则可在4小时内完成测试。通过滴定病毒原种确定的B-A ELISA的检出限为每50微升病毒原种大约90 PFU或HSV-1或HSV-2的6 X 10（3）个物理颗粒。在一项研究中获得了以下结果，在该研究中，从各种病变中抽取了拭子，并通过B-A ELISA测定了组织培养物中的感染性。在421例疑似HSV病变中，两项检测均为阳性，两种检测均为159阴性。通过B-A ELISA共有122例阳性，但感染性阴性。 B-A ELISA检测结果为71阴性，但含有传染性病毒。通过部分阻断与兔免疫球蛋白G抗HSV的反应性以及不与非特异性生物素标记的小鼠免疫球蛋白M单克隆抗体的反应性，证实了该检测方法的HSV特异性。

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