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首页> 外文期刊>Journal of Clinical Microbiology >Viral antibody screening system that uses a standardized single dilution immunoglobulin G enzyme immunoassay with multiple antigens.
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Viral antibody screening system that uses a standardized single dilution immunoglobulin G enzyme immunoassay with multiple antigens.

机译：病毒抗体筛选系统，该系统使用具有多个抗原的标准化单稀释免疫球蛋白G酶免疫测定。

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We present an enzyme-linked immunosorbent assay (ELISA) system for the simultaneous determination of immunoglobulin G antibodies directed against several viruses. Antibodies to up to eight different viruses could be determined for three different sera on one microtitration plate. After subtraction of the absorbance values obtained with the control antigens, the viral antigen absorbancies were expressed as percentages of the absorbance obtained with a pooled immunoglobulin standard. This value, the relative antibody activity, was rapidly calculated by means of a computer directly connected to the ELISA photometer and was stored on magnetic disks, thereby facilitating seroepidemiological studies. The reproducibility of the relative antibody activity was calculated to at best +/- 3.6% (standard deviation) in an intraassay test and to at worst +/- 20.4% (standard deviation) in an interassay test. Each serum was analyzed only at a dilution of 1/75. The sensitivity of this single-dilution ELISA (SD-ELISA) method for the detection of titer rises was compared with those of conventional methods, mostly complement fixation but also hemagglutination inhibition. A total of 142 of 155 (92%) paired sera showing fourfold complement fixation or hemagglutination inhibition rises also showed significant results in SD-ELISA. A total of 22 of 57 (39%) significant relative antibody activity rises were significant in complement fixation or hemagglutination inhibition. Overall, up to twice as many significant titer rises could be detected with SD-ELISA. Most of these seemed to have a sound correlation with clinical data. The specificity of SD-ELISA was found to be similar to that of complement fixation, with some cross-reactions occurring between herpes simplex and varicella-zoster virus antigens and between parainfluenza viruses. We have found SD-ELISA to be a valuable clinical virological tool that supplements conventional serology.
机译：我们提出了一种同时测定针对几种病毒的免疫球蛋白G抗体的酶联免疫吸附测定（ELISA）系统。可以在一个微量滴定板上针对三种不同的血清确定多达八种不同病毒的抗体。减去用对照抗原获得的吸光度值后，病毒抗原的吸光度表示为用合并的免疫球蛋白标准液获得的吸光度的百分比。该值，即相对抗体活性，是通过直接连接到ELISA光度计的计算机快速计算的，并存储在磁盘上，从而促进了血清流行病学研究。相对抗体活性的重现性在测定内测试中计算为最佳+/- 3.6％（标准偏差），在测定间测试中计算为最差+/- 20.4％（标准偏差）。仅以1/75的稀释度分析每种血清。将这种单稀释ELISA（SD-ELISA）方法检测滴度升高的灵敏度与常规方法（主要是补体固定但还具有血凝抑制作用）进行了比较。 155个配对血清中的142个（占92％）配对血清显示出四倍的补体固定或血凝抑制抑制作用，在SD-ELISA中也显示了显着结果。在57个相对抗体活性的显着升高中，总共有22个（39％）在补体固定或血凝抑制方面显着。总体而言，使用SD-ELISA可以检测到多达两倍的显着滴度升高。其中大多数似乎与临床数据具有良好的相关性。发现SD-ELISA的特异性与补体固定的特异性相似，在单纯疱疹病毒和水痘带状疱疹病毒抗原之间以及副流感病毒之间发生一些交叉反应。我们发现SD-ELISA是补充常规血清学的有价值的临床病毒学工具。

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