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首页> 外文期刊>Journal of Clinical Microbiology >Evaluation of the Quantum II and Rapid E identification systems.
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Evaluation of the Quantum II and Rapid E identification systems.

机译:评估Quantum II和Rapid E识别系统。

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A total of 492 clinical isolates from the family Enterobacteriaceae were tested in the API 20E, Rapid E, and Quantum II identification systems. Discrepant identifications among these three systems were resolved by repeat testing in the identification systems or use of conventional biochemical tests. Of these isolates, 94.1% were correctly identified with the API 20E and Rapid E systems, and 97.0% were correctly identified with the Quantum II system. An additional 48 non-Enterobacteriaceae isolates were tested with the Quantum II system, and 83.3% were correctly identified. The majority of incorrect identifications with the Rapid E and Quantum II systems were caused by a single aberrant biochemical reaction. Reproducibility of the biochemical reactions obtained with these two systems was evaluated by testing 40 organisms in triplicate. Identical biocodes for all three tests were obtained for 10 organisms with the Quantum II system and for 19 organisms with the Rapid E system. Reproducibility of the Quantum II test results was improved with a subsequent modification of the photometer of this system. Both the Rapid E and Quantum II systems were inexpensive and were technically easy to inoculate and interpret.
机译:在API 20E,Rapid E和Quantum II鉴定系统中对来自肠杆菌科的总共492个临床分离株进行了测试。通过在识别系统中进行重复测试或使用常规生化测试,可以解决这三个系统中的识别差异。在这些分离物中,使用API​​ 20E和Rapid E系统正确鉴定出94.1%,使用Quantum II系统正确鉴定出97.0%。使用Quantum II系统测试了另外48个非肠杆菌科细菌分离物,正确鉴定出83.3%。使用Rapid E和Quantum II系统进行的大多数错误识别都是由单个异常的生化反应引起的。通过一式三份测试40个生物,评估了通过这两个系统获得的生化反应的可重复性。使用Quantum II系统获得的10种生物体和使用Rapid E系统获得的19种生物体均获得了所有三种测试的相同生物密码。后来修改了该系统的光度计,提高了Quantum II测试结果的可重复性。 Rapid E和Quantum II系统都很便宜,并且在技术上易于接种和解释。

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