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首页> 外文期刊>Journal of Clinical Microbiology >Development of a DNA probe to detect Salmonella typhi.
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Development of a DNA probe to detect Salmonella typhi.

机译：开发一种DNA探针以检测伤寒沙门氏菌。

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摘要

This study was undertaken to identify a DNA sequence that could be used to facilitate the diagnostic identification of Salmonella typhi, the causative agent of typhoid fever. All virulent S. typhi strains encode a relatively unique capsular antigen termed the virulence (Vi) antigen. Two distinct genetic loci, viaA and viaB, are involved in the synthesis of this antigen. The structural genes, located at viaB, were considered as a possible specific DNA probe. The viaB locus, contained in a recombinant cosmid, was subcloned to various plasmid vectors for this purpose. Selected viaB-region DNA fragments were then analyzed for specificity in DNA colony hybridization reactions with more than 170 strains representing a variety of enteric bacteria. An 8.6-kilobase EcoRI fragment was highly specific for the viaB gene region and was considered a good hybridization probe. This DNA probe should prove useful in rapid diagnostic assays set up to detect S. typhi in mixed bacterial samples (e.g., stools) within a few hours of specimen collection.
机译：进行这项研究以鉴定可用于促进伤寒沙门氏菌（伤寒的病原体）的诊断鉴定的DNA序列。所有有毒的伤寒链球菌菌株都编码一个相对独特的荚膜抗原，称为毒力（Vi）抗原。该抗原的合成涉及两个不同的遗传基因座，viaA和viaB。位于viaB的结构基因被认为是可能的特异性DNA探针。为此，将包含在重组粘粒中的viaB基因座亚克隆到各种质粒载体中。然后，通过与代表多种肠细菌的170多种菌株进行的DNA菌落杂交反应，分析了选定的viaB区域DNA片段的特异性。 8.6碱基对的EcoRI片段对viaB基因区域具有高度特异性，被认为是良好的杂交探针。该DNA探针应被证明可用于快速诊断检测，该检测可在标本采集后的几个小时内检测出混合细菌样品（例如粪便）中的伤寒沙门氏菌。

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