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首页> 外文期刊>Journal of Clinical Microbiology >Evaluation of mannitol salt agar with oxacillin as a screening medium for methicillin-resistant Staphylococcus aureus.
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Evaluation of mannitol salt agar with oxacillin as a screening medium for methicillin-resistant Staphylococcus aureus.

机译：用奥沙西林作为耐甲氧西林金黄色葡萄球菌的筛选培养基评估甘露醇盐琼脂。

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We evaluated the use of mannitol salt agar with oxacillin for use as a primary screening medium for the simultaneous detection and identification of methicillin-resistant Staphylococcus aureus in clinical surveillance specimens. Oxacillin agar dilution susceptibility tests with mannitol salt agar and Mueller-Hinton agar were performed in parallel with disk-agar diffusion testing on 95 oxacillin-susceptible and 105 oxacillin-resistant S. aureus stock isolates. MICs were found to be comparable, showing distinct separation of susceptible and resistant isolates into two groups with MICs of less than or equal to 2 and greater than or equal to 32 micrograms/ml, respectively. In accord with these findings, 4 micrograms of oxacillin per ml was selected for use in the screening medium. For performance evaluation, mannitol salt agar with 4 micrograms of oxacillin per ml was compared with mannitol salt agar without oxacillin by performing parallel screening tests on 153 clinical surveillance specimens. For detection of methicillin-resistant S. aureus, mannitol salt agar with 4 micrograms of oxacillin per ml was as sensitive as mannitol salt agar without oxacillin and required significantly fewer confirmatory tests. For primary identification of methicillin-resistant S. aureus, mannitol salt agar with 4 micrograms of oxacillin per ml was 6.4% false-positive and 1.1% false-negative, with a 93.6% positive predictive value. These findings indicate that mannitol salt agar with 4 micrograms of oxacillin per ml can be used as a reliable and cost-effective screening medium for the simultaneous detection and identification of methicillin-resistant S. aureus in clinical surveillance specimens.
机译：我们评估了将甘露醇盐琼脂和奥沙西林用作同时监测和鉴定临床监测标本中耐甲氧西林的金黄色葡萄球菌的主要筛选介质。与甘露醇盐琼脂和Mueller-Hinton琼脂一起进行的奥沙西林琼脂稀释敏感性测试与对95例对奥沙西林敏感和105例耐奥西西林的金黄色葡萄球菌分离株的圆盘琼脂扩散测试同时进行。发现MIC是可比较的，显示出敏感和抗性分离物明显分为两组，MIC分别小于或等于2和大于或等于32微克/毫升。根据这些发现，筛选培养基中每毫升选择了4微克的奥沙西林。为了进行性能评估，通过对153个临床监测标本进行平行筛选试验，将每毫升含4微克奥沙西林的甘露醇盐琼脂与不含奥沙西林的甘露醇盐琼脂进行比较。为了检测耐甲氧西林的金黄色葡萄球菌，每毫升含4微克奥沙西林的甘露醇盐琼脂与不含奥沙西林的甘露醇盐琼脂一样灵敏，所需的确证试验要少得多。为了初步鉴定耐甲氧西林的金黄色葡萄球菌，每毫升含4微克奥沙西林的甘露醇盐琼脂的假阳性率为6.4％，假阴性的为1.1％，阳性预测值为93.6％。这些发现表明，每毫升含4微克奥沙西林的甘露醇盐琼脂可用作可靠且具有成本效益的筛选介质，用于同时检测和鉴定临床监测标本中耐甲氧西林的金黄色葡萄球菌。

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