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首页> 外文期刊>Journal of Clinical Microbiology >Spheroplast induction in clinical isolates of Serratia marcescens in the presence of Ca2+ or Mg2+.
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Spheroplast induction in clinical isolates of Serratia marcescens in the presence of Ca2+ or Mg2+.

机译：在Ca2 +或Mg2 +存在下，粘质沙雷氏菌临床分离株中的原生质球诱导。

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Serratia marcescens was easily induced to form spheroplasts by beta-lactam antibiotics in the presence of Ca2+ or Mg2+ without an osmotic stabilizer such as sucrose. The spheroplasts grew in volume, although they could not divide. They were stable for more than 10 h at 37 degrees C in a medium containing a high concentration of antibiotic, and they had the ability to revert to the original bacillary form. Ca2+ was more effective in spheroplast induction than Mg2+. The effect was proportional to the concentration of cations. In 40% of 180 clinical isolates of S. marcescens, more than 40% of the original bacterial cells were induced to form spheroplasts by ceftizoxime in a medium supplemented with 40 mM Ca2+. A high spheroplast induction rate was observed even in medium with 10 mM Ca2+. Few isolates that were supersusceptible to ceftizoxime (MIC, less than 0.2 microgram/ml) were induced to form spheroplasts at a high rate. No difference in spheroplast induction rate or extent between antibiotic-resistant strains and relatively susceptible strains (MIC, greater than 0.2 microgram/ml) was found. The serotype of S. marcescens had no effect on the spheroplast induction rate. Monocations (Na+ and K+) had little effect on spheroplast induction.
机译：在无渗透稳定剂（如蔗糖）的Ca2 +或Mg2 +存在下，β-内酰胺类抗生素很容易诱导粘质沙雷氏菌形成原生质球。原生质球的体积增长，尽管它们不能分裂。在含有高浓度抗生素的培养基中，它们在37摄氏度下可稳定10小时以上，并且具有恢复原始细菌形式的能力。 Ca2 +在原生质球诱导方面比Mg2 +更有效。效果与阳离子浓度成正比。在180种Marcescens的临床分离株中，有40％由头孢唑肟在补充40 mM Ca2 +的培养基中诱导超过40％的原始细菌细胞形成原生质球。即使在含有10 mM Ca2 +的培养基中也观察到了很高的原生质球诱导率。极少数对头孢唑肟易感的分离株（MIC，小于0.2微克/毫升）被诱导以高速率形成原生质球。在抗生素抗性菌株和相对易感菌株（MIC，大于0.2微克/ ml）之间未发现原生质球诱导速率或程度的差异。粘质链球菌的血清型对原生质球诱导率没有影响。单阳离子（Na +和K +）对原生质球的诱导作用很小。

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