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首页> 外文期刊>Journal of Clinical Microbiology >Potential use of continuous cell lines to distinguish between pathogenic and nonpathogenic Listeria spp.
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Potential use of continuous cell lines to distinguish between pathogenic and nonpathogenic Listeria spp.

机译:潜在使用连续细胞系区分致病性李斯特菌和非致病性李斯特菌。

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Continuous cell lines were tested for their potential use in distinguishing pathogenic from nonpathogenic Listeria species. Listeria monocytogenes and Listeria ivanovii strains were lethal for mice, and culture filtrates were cytotoxic for cultured cells and hemolytic for sheep erythrocytes, while Listeria innocua, Listeria grayi, and Listeria murrayi strains were negative in all three tests. The eight cell lines tested were all affected but varied in sensitivity, with the Chinese hamster ovary cell line being the most sensitive. Cytolytic effect was noted within minutes of the addition of undiluted filtrates, with optimum titers obtained by 24 h.
机译:测试了连续细胞系在区分致病性李斯特菌和非致病性李斯特菌中的潜在用途。单核细胞增生李斯特菌和伊万氏李斯特菌菌株对小鼠具有致死性,培养滤液对培养细胞具有细胞毒性,对绵羊红细胞具有溶血作用,而无毒李斯特菌,灰色李斯特菌和墨累李斯特菌菌株在所有三个测试中均为阴性。测试的八种细胞系均受影响,但敏感性不同,其中中国仓鼠卵巢细胞系最敏感。加入未稀释的滤液后几分钟内即可观察到细胞溶解作用,并在24小时内获得最佳效价。

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