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首页> 外文期刊>Journal of Clinical Microbiology >Comparison of primary rabbit kidney and MRC-5 cells and two stain procedures for herpes simplex virus detection by a shell vial centrifugation method.
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Comparison of primary rabbit kidney and MRC-5 cells and two stain procedures for herpes simplex virus detection by a shell vial centrifugation method.

机译：比较原代兔肾细胞和MRC-5细胞以及两种通过贝壳小瓶离心法检测单纯疱疹病毒的染色方法。

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By using a conventional tissue culture method as a standard, four shell vial centrifugation culture (SVC) formats were compared for herpes simplex virus (HSV) detection in 300 clinical samples. Both MRC-5 and primary rabbit kidney (PRK) cells were used in the conventional and SVC systems. In addition, both a direct monoclonal fluorescent antibody to HSV (MAb-FA; Syva Corporation, Palo Alto, Calif.) and an indirect HSV polyclonal antibody-horseradish peroxidase stain (poly-HRP; Difco Laboratories, Detroit, Mich.) were used to stain shell vials of both cell types. Conventional tubes were incubated for up to 7 days with daily examination for cytopathic effect, which was confirmed as HSV by staining with an MAb-FA. Shell vials were inoculated, centrifuged, incubated for 16 to 24 h, and stained directly with MAb-FA or indirectly with a poly-HRP stain. Of the 300 specimens examined, 82 (27%) were HSV positive by conventional tissue culture. PRK cells detected 81 (99%) positive specimens, compared with 74 (90%) specimens detected with MRC-5 cells. Of the 82 positive specimens by conventional culture, the SVC formats detected 68 by MRC-5 and MAb-FA, 74 by MRC-5 and poly-HRP, 64 by PRK and MAb-FA, and 77 by PRK and poly-HRP. Therefore, PRK stained by an indirect method with poly-HRP was the most sensitive of the SVC formats tested, detecting 94% of the positive specimens.
机译：通过使用常规的组织培养方法作为标准，在300个临床样品中比较了四种壳小瓶离心培养（SVC）格式的单纯疱疹病毒（HSV）检测。在常规和SVC系统中都使用了MRC-5和原代兔肾（PRK）细胞。此外，使用了针对HSV的直接单克隆荧光抗体（MAb-FA; Syva Corporation，帕洛阿尔托，加利福尼亚州）和间接HSV多克隆抗体-辣根过氧化物酶染色剂（poly-HRP; Difco Laboratories，底特律，密歇根州）。对两种细胞类型的贝壳小瓶染色。每天将常规试管孵育7天，每天检查其细胞病变效应，并通过MAb-FA染色确认为HSV。接种，离心壳小瓶16至24小时，然后直接用MAb-FA染色或间接用poly-HRP染色。通过常规组织培养，在检查的300个样本中，有82个（27％）为HSV阳性。 PRK细胞检测到81个（99％）阳性标本，而MRC-5细胞检测到74个（90％）标本。在常规培养的82份阳性样本中，SVC格式通过MRC-5和MAb-FA检测出68种，通过MRC-5和poly-HRP检测出74种，通过PRK和MAb-FA检测出64种，通过PRK和poly-HRP检测出77种。因此，用多HRP间接方法对PRK染色是所测试的SVC格式中最敏感的，检测到94％的阳性样本。

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