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首页> 外文期刊>Journal of Clinical Microbiology >Adaptation of plastic surfaces for tissue culture by glow discharge.
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Adaptation of plastic surfaces for tissue culture by glow discharge.

机译：通过辉光放电使塑料表面适应组织培养。

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Plastic petri dishes and microtitration plates were electrically charged by a glow discharge unit installed in a vacuum evaporator. Charged and uncharged plates, as well as plates commercially treated for tissue culture, were inoculated with Vero and BHK-21 cell lines; secondary cultures of monkey kidney, chicken lung, canine kidney, and embryonic bovine kidney; and primary chicken embryo fibroblasts and chicken lung cells. All cell cultures grew normally on petri plates charged with the covers open. Growth rate and cell density compared favorably with growth on the commercial tissue culture plates; cell growth was somewhat less dense, however, on plates charged with the covers closed. Charged plates could be sterilized by ultraviolet light and ethylene oxide with no adverse effects on cell growth. Cells inoculated onto plates charged up to 7 months before inoculation grew as well as on freshly charged plates.
机译：塑料陪替氏培养皿和微量滴定板通过安装在真空蒸发器中的辉光放电单元充电。用Vero和BHK-21细胞系接种带电荷的板和不带电荷的板，以及商业处理过的用于组织培养的板。猴肾，鸡肺，犬肾和胚胎牛肾的继代培养;和原代鸡胚成纤维细胞和鸡肺细胞。所有细胞培养物均在装有盖的培养皿上正常生长。生长速度和细胞密度优于商业组织培养板上的生长;然而，在封闭盖的板上，细胞的生长密度稍低。带电板可通过紫外线和环氧乙烷灭菌，对细胞生长无不利影响。接种到接种前长达7个月的已充电平板上的细胞以及新鲜充电的平板上。

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