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首页> 外文期刊>Journal of Clinical Microbiology >Comparison of the reversed passive hemagglutination with radioimmunoassay methods for hepatitis B antigen.
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Comparison of the reversed passive hemagglutination with radioimmunoassay methods for hepatitis B antigen.

机译：反向被动血凝法与放射免疫法对乙型肝炎抗原的比较。

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Radioimmunoassay for the detection of hepatitis B antigen has been accepted in many diagnostic laboratories now. The question of nonspecific positives has always been a matter of controversy. Two improved radioimmunoassay tests, namely Ausria II-125 by Abbott Laboratories and a radioimmunoassay method by Connaught Laboratories Limited (Hebria), were compared with the original Ausria 125I. Included in the comparison was the reversed passive hemagglutination test (Auscell, Abbott). Five hundred sera of clinical patients were tested. Fifty-five or 11% were found to have hepatitis B antigen. Three tests, Ausria 125I, Ausria II-125, and Hebria showed the same number of positive sera, whereas Auscell missed one. However, Ausria 125I indicated two additional false positives. Dilution experiments, however, indicated that Ausria II-125 and Hebria were the most sensitive tests, with the reversed passive hemaglutination showing the least sensitivity. Therefore, the new Ausria II-125 and the Hebria radioimmunoassay tests are preferable in view of their sensitivity and specificity.
机译：现在，许多诊断实验室已经接受了用于检测乙型肝炎抗原的放射免疫分析法。非特异性阳性问题一直是一个有争议的问题。将两个改进的放射免疫分析测试，即雅培公司的Ausria II-125和康诺特实验室有限公司（希伯利亚）的放射免疫方法与原始的澳大利亚125I进行了比较。比较中包括反向被动血凝试验（Auscell，Abbott）。测试了500例临床患者的血清。发现其中百分之五十五或百分之十一具有乙型肝炎抗原。 Ausria 125I，Ausria II-125和Hebria这三项测试显示出相同数量的阳性血清，而Auscell却漏掉了一份。但是，Ausria 125I表示另外两个误报。然而，稀释实验表明，Ausria II-125和Hebria是最敏感的测试，反向被动血凝检测显示出最低的敏感度。因此，考虑到它们的敏感性和特异性，新的Ausria II-125和Hebria放射免疫分析法是优选的。

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