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首页> 外文期刊>Journal of Clinical Microbiology >Fermentation of raffinose by lactose-fermenting strains of Yersinia enterocolitica and by sucrose-fermenting strains of Escherichia coli.
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Fermentation of raffinose by lactose-fermenting strains of Yersinia enterocolitica and by sucrose-fermenting strains of Escherichia coli.

机译：通过小肠结肠炎耶尔森氏菌的乳糖发酵菌株和大肠杆菌的蔗糖发酵菌株对棉子糖的发酵。

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摘要

Introduction of plasmids carrying the lacY gene (lactose permease gene) into Yersinia enterocolitica results in cells being able to ferment both lactose and raffinose. Transfer of such plasmids into Escherichia coli C600 (lacY) confers ability to ferment lactose but not raffinose. Derivatives of C600 that ferment both lactose and sucrose (Lac+ Scr+ strains) are able to ferment raffinose, but do not grow well on raffinose minimal medium. Fermentation of raffinose by Lac+ strains of Y. enterocolitica, and by Lac+ Scr+ strains of E. coli, is explained in terms of transport of raffinose via the lac permease and subsequent breakdown catalyzed by invertase.
机译：将携带lacY基因（乳糖通透酶基因）的质粒导入小肠结肠炎耶尔森氏菌，可以使细胞发酵乳糖和棉子糖。将此类质粒转移到大肠杆菌C600（lacY）中可赋予发酵乳糖而非棉子糖的能力。同时发酵乳糖和蔗糖的C600衍生物（Lac + Scr +菌株）能够发酵棉子糖，但在棉子糖基本培养基上不能很好地生长。就棉子糖经由lac渗透酶的运输以及随后被转化酶催化的降解而言，解释了由小肠结肠炎耶尔森氏菌的Lac +菌株和大肠杆菌的Lac + Scr +菌株对棉子糖的发酵。

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