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首页> 外文期刊>Journal of Clinical Microbiology >Sensitive chemiluminescent enzyme-linked immunosorbent assay for quantification of human immunoglobulin G and detection of herpes simplex virus.
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Sensitive chemiluminescent enzyme-linked immunosorbent assay for quantification of human immunoglobulin G and detection of herpes simplex virus.

机译：灵敏的化学发光酶联免疫吸附测定法，用于定量人免疫球蛋白G和检测单纯疱疹病毒。

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摘要

A chemiluminescent enzyme-linked immunosorbent assay (CELISA) was developed for detecting human immunoglobulin G and herpes simplex viral antigen. A comparison of CELISA with a conventional absorptiometric detection system showed that CELISA was 100 times more sensitive than absorptiometry for the measurement of human immunglobulin G. Similarly, CELISA detected as few as 40 plaque-forming units of herpes simplex virus in contrast to 2,500 plaque-forming units detected by absorptiometry. Of 18 specimens which were positive for herpes simplex virus type 1 by isolation in tissue culture, 15 (83%) were detected by CELISA within a few hours; in certain cases, several days were necessary for detection of virus by isolation techniques.
机译：建立了一种化学发光酶联免疫吸附测定法（CELISA），用于检测人免疫球蛋白G和单纯疱疹病毒抗原。 CELISA与常规吸光度检测系统的比较表明，对于人类免疫球蛋白G的测量，CELISA的灵敏度比吸光度高100倍。同样，CELISA检测到只有40个斑块形成单位的单纯疱疹病毒，而2500个斑块吸光光度法检测形成单位。通过组织培养分离得到的18例1型单纯疱疹病毒阳性的标本中，在几个小时内通过CELISA检测到15例（83％）。在某些情况下，通过隔离技术检测病毒需要几天的时间。

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