Detection of La Crosse arbovirus antigen in mosquito pools: application of chromogenic and fluorogenic enzyme immunoassay systems.

S W Hildreth; B J Beaty; J M Meegan; C L Frazier; R E Shope

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Detection of La Crosse arbovirus antigen in mosquito pools: application of chromogenic and fluorogenic enzyme immunoassay systems.

机译：在蚊子池中检测La Crosse虫媒病毒抗原：发色和荧光酶免疫测定系统的应用。

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An enzyme immunoassay producing either a chromogenic or fluorogenic end product was developed and evaluated for detecting La Crosse viral antigen within mosquito pools. The enzyme immunoassay was found to be sensitive, detecting one infected mosquito within a pool of 100 mosquitoes, and specific, distinguishing between closely related California group viruses. Assays were completed within 5 h after the addition of test samples. La Crosse viral antigen could be readily detected in mosquito pools after seven freeze-thaw cycles.

机译：开发了一种产生发色或发荧光终产物的酶免疫测定法，并进行了评估，以检测蚊池中的拉克罗斯病毒抗原。发现该酶免疫测定法灵敏，可在100个蚊子池中检测到一只被感染的蚊子，并且具有特异性，可区分密切相关的加利福尼亚群病毒。在添加测试样品后5小时内完成测定。在七个冻融循环后，蚊子池中很容易检测到La Crosse病毒抗原。

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《Journal of Clinical Microbiology》 |1982年第5期|共6页
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S W Hildreth; B J Beaty; J M Meegan; C L Frazier; R E Shope;
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中图分类医学微生物学（病原细菌学、病原微生物学）;
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6. Detection of La Crosse arbovirus antigen in mosquito pools: application of chromogenic and fluorogenic enzyme immunoassay systems. [O] . S W Hildreth, B J Beaty, J M Meegan, 1982

机译：在蚊子池中检测La Crosse虫媒病毒抗原：发色和荧光酶免疫测定系统的应用。
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8. Prospective Field Evaluation of an Enzyme Immunoassay: Detection of Eastern Equine Encephalomyelitis Virus Antigen in Pools of Culiseta Melanura [R] . Scott, T. W., Olson, J. G., Lewis, T. E., 1987

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Detection of La Crosse arbovirus antigen in mosquito pools: application of chromogenic and fluorogenic enzyme immunoassay systems.

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