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首页> 外文期刊>Journal of Clinical Microbiology >Evaluation of rapid SYS system as screen for Yersinia enterocolitica in the United States.
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Evaluation of rapid SYS system as screen for Yersinia enterocolitica in the United States.

机译：在美国评估快速SYS系统作为小肠结肠炎耶尔森氏菌的筛选方法。

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Clinical isolates (n = 150) from stool specimens were selected for evaluation of the Rapid SYS system (Analytab Products, Plainview, N.Y.) as a screening test for Shigella spp., Yersinia enterocolitica, and Salmonella spp. The Gram-Negative Identification Card (Vitek Systems, Inc., Hazelwood, Mo.) was used for identification. Although acceptable performance of the Rapid SYS system was described, the interpretative criteria provided by the vendor for previous studies led to inappropriate screening for Y. enterocolitica, particularly biotype 1. When corrected screening criteria were used for the present study, the sensitivity for the detection of 76 enteric pathogens was 98.7%. Of the 76 pathogens, 1 of 21 Shigella spp. was not detected. However, specificity was only 16.6% when 72 selected nonpathogens frequently encountered in stools were eliminated. Although the Rapid SYS system can identify Shigella spp., Y. enterocolitica, and Salmonella spp., only phenylalanine deaminase-producing and cytochrome oxidase-producing organisms can be eliminated from additional testing. Therefore, the Rapid SYS system cannot be used as a three-pathogen screen in the United States or in other geographic locales where Y. enterocolitica biotype 1 may be encountered.
机译：选择粪便标本中的临床分离株（n = 150）进行Rapid SYS系统（Analytab Products，Plainview，N.Y.）的评估，以作为对志贺氏菌，小肠结肠炎耶尔森氏菌和沙门氏菌的筛选试验。革兰氏阴性识别卡（Vitek Systems，Inc.，Hazelwood，MO）用于识别。尽管描述了Rapid SYS系统可接受的性能，但供应商为以前的研究提供的解释性标准导致对肠球菌耶尔森菌，特别是生物型1的筛查不当。当本研究使用校正的筛查标准时，检测的敏感性76种肠道病原体占98.7％。在76种病原体中，有21种志贺氏菌属。未检测到。但是，当排除了72种在粪便中经常遇到的非病原体时，特异性仅为16.6％。尽管Rapid SYS系统可以识别志贺氏菌，小肠结肠炎耶尔森氏菌和沙门氏菌，但只有苯丙氨酸脱氨酶产生菌和细胞色素氧化酶产生菌可以从其他测试中消除。因此，在美国或可能遇到小肠结肠炎耶尔森氏菌生物型1的其他地理区域中，Rapid SYS系统无法用作三病原体筛查。

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