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首页> 外文期刊>Journal of Clinical Microbiology >Plasmid analysis as a means of strain differentiation in Clostridium perfringens.
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Plasmid analysis as a means of strain differentiation in Clostridium perfringens.

机译：质粒分析作为产气荚膜梭菌中菌株分化的一种手段。

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A total of 114 Clostridium perfringens isolates were serotyped and examined for plasmids. Fifty-two strains were from hospitalized patients with diarrhea or from hospital environments, and 62 epidemiologically unrelated isolates were obtained from food poisoning outbreaks. All strains were screened for bacteriocin production against a common indicator strain of C. perfringens. In the one significant hospital outbreak of C. perfringens diarrhea, three to five plasmid types were found in strains of the predominant serotype, but no similar correlation between serotype and plasmid type was found in random isolates from a variety of sources. All of the strains associated with the diarrhea outbreak produced bacteriocins, whereas 63% of the strains from various sources produced bacteriocins. The typing data suggest a promising differentiating capability for plasmid analysis in the epidemiological study of outbreaks of food poisoning, diarrhea, or infections caused by C. perfringens.
机译：对总共114种产气荚膜梭菌分离物进行血清分型并检查质粒。 52株来自腹泻住院患者或医院环境，从食物中毒暴发中获得了62种与流行病学无关的分离株。针对产气荚膜梭菌的普通指示菌株筛选所有菌株的细菌素产生。在一次产气荚膜梭菌腹泻的重大医院暴发中，在主要血清型菌株中发现了三到五种质粒类型，但是在来自各种来源的随机分离株中，血清型和质粒类型之间没有相似的相关性。与腹泻暴发有关的所有菌株均产生细菌素，而来自各种来源的63％的菌株均产生细菌素。打字数据表明在流行性食物中毒，腹泻或产气荚膜梭菌引起的感染的流行病学研究中，质粒分析有希望的区分能力。

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