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首页> 外文期刊>Journal of Clinical Microbiology >Rapid identification of obligately anaerobic gram-positive cocci using high-performance liquid chromatography.
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Rapid identification of obligately anaerobic gram-positive cocci using high-performance liquid chromatography.

机译：使用高效液相色谱法快速鉴定专性厌氧革兰氏阳性球菌。

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High-performance liquid chromatography was evaluated as a rapid means of identifying obligately anaerobic gram-positive cocci of medical interest. Isolates were inoculated into a defined chemical medium consisting primarily of amino acids and were incubated aerobically for 1 h at 35 degrees C. After removal of organisms, the supernatant fluids were derivatized for 1 min at room temperature by the addition of o-phthalaldehyde. The total time required to run a chromatogram was approximately 50 min. Standardized peak heights for each medium component and any new peaks formed were calculated for each isolate and compared with those for uninoculated control medium. Multiple isolates of various species of anaerobic gram-positive cocci gave consistent patterns of medium utilization that could be used for identification purposes. This method can easily be adapted for laboratory use and has the potential for automated microbial identification.
机译：高效液相色谱法被评估为鉴定医学上专性厌氧革兰氏阳性球菌的快速方法。将分离物接种到主要由氨基酸组成的特定化学培养基中，并在35摄氏度有氧条件下孵育1小时。去除生物后，通过添加邻苯二甲醛在室温下将上清液衍生1分钟。运行色谱图所需的总时间约为50分钟。计算每种分离物的每种培养基成分的标准峰高和形成的任何新峰，并将其与未接种的对照培养基进行比较。多种厌氧革兰氏阳性球菌的多重分离物提供了一致的培养基利用模式，可用于鉴定目的。这种方法很容易适合实验室使用，并具有自动鉴定微生物的潜力。

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