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首页> 外文期刊>Journal of Clinical Microbiology >Reactivity of human Lyme borreliosis sera with a 39-kilodalton antigen specific to Borrelia burgdorferi.
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Reactivity of human Lyme borreliosis sera with a 39-kilodalton antigen specific to Borrelia burgdorferi.

机译：人莱姆疏螺旋体症血清与伯氏疏螺旋体特异的39千达尔顿抗原的反应性。

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Borrelia burgdorferi is the causative agent of Lyme borreliosis, a spirochetal illness with a variety of acute clinical manifestations that may lead to debilitating neurological and arthritic complications. Diagnosis is difficult because symptoms mimic a variety of unrelated clinical conditions, spirochetes cannot always be isolated from infected patients, and current serological tests are frequently inconclusive because of the presence of cross-reacting non-B. burgdorferi antibodies. To identify antigens specific to B. burgdorferi that could be used in the serodiagnosis of Lyme borreliosis, we screened a Borrelia DNA expression library in Escherichia coli for antigens reactive with human Lyme borreliosis sera. One clone carried a 6.3-kilobase EcoRI chromosomal fragment (pSPR33), which encoded two species-specific antigens with molecular masses of 28 (P28) and 39 (P39) kilodaltons (kDa). These two antigens were immunologically distinct from OspA, OspB, and the 41-kDa flagellin. Ninety-four serum specimens from patients having Lyme borreliosis were tested for reactivity with P39. All of 33 the serum specimens with immunofluorescence assay titers of greater than or equal to 1:256, 13 of 17 serum specimens with titers of 1:128, and 14 of 44 serum specimens with titers of less than or equal to 1:64 reacted with P39. Notably, many sera reactive to P39 did not appear to react with the 41-kDa flagellin. Therefore, antibody to P39 could be mistaken for antibody to the 41-kDa flagellin in tests of human sera by Western blot (immunoblot). Twenty-five control serum specimens, which included sera from syphilitic, relapsing fever, and amyotrophic lateral sclerosis patients as well as from 10 normal individuals, did not react to P39. Our data suggest that P39 may be a useful antigen for the serological confirmation of Lyme borreliosis.
机译：伯氏疏螺旋体是莱姆氏疏螺旋体病的病原体，莱姆氏疏螺旋体病是一种螺旋体疾病，具有多种急性临床表现，可能导致虚弱的神经系统和关节炎并发症。诊断很困难，因为症状模仿各种不相关的临床状况，螺旋体不能总是从感染的患者中分离出来，并且由于存在交叉反应的非B抗原，目前的血清学检测常常是不确定的。伯氏抗体。为了鉴定可用于伯氏莱姆病血清学诊断的B. burgdorferi特异抗原，我们在大肠杆菌中筛选了与人莱姆氏疏螺旋体血清反应的抗原的伯氏疏螺旋体DNA表达文库。一个克隆带有一个6.3碱基碱基的EcoRI染色体片段（pSPR33），该片段编码两个物种特异性抗原，分子量分别为28（P28）和39（P39）千道尔顿（kDa）。这两种抗原在免疫学上不同于OspA，OspB和41-kDa鞭毛蛋白。测试了患有莱姆病的患者的94份血清标本与P39的反应性。免疫荧光测定滴度大于或等于1：256的33个血清样本，滴度小于1：128的17个血清样本中的13个和滴度小于或等于1:64的44个血清样本中的14个反应了与P39。值得注意的是，许多对P39具有反应性的血清似乎未与41-kDa鞭毛蛋白反应。因此，在人血清检测中，通过蛋白质印迹法（免疫印迹）可将针对P39的抗体误认为是针对41 kDa鞭毛蛋白的抗体。 25份对照血清标本对P39无反应，其中包括梅毒，复发性发热和肌萎缩性侧索硬化患者的血清以及10例正常人的血清。我们的数据表明，P39可能是有用的抗原，可用于血清学确认为Lyme borreliosis。

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