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首页> 外文期刊>Journal of Clinical Microbiology >Preliminary evaluation of Biolog, a carbon source utilization method for bacterial identification.
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Preliminary evaluation of Biolog, a carbon source utilization method for bacterial identification.

机译：初步评估Biolog，一种用于细菌鉴定的碳源利用方法。

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The Biolog Identification System (Biolog, Inc., Hayward, Calif.) is a new bacterial identification method that establishes an identification based on the exchange of electrons generated during respiration, leading to a subsequent tetrazolium-based color change. This system tests the ability of a microorganism to oxidize a panel of 95 different carbon sources. We report on a preliminary investigation of the ability of the instrument to identify, using its computer-driven enzyme immunoassay reader, a diverse group of clinically relevant members of the family Enterobacteriaceae and gram-negative non-Enterobacteriaceae. The Biolog reported identifications (correct or incorrect) for 266 of 352 organisms tested (75.6%). Of the 266 identifications reported, 87.3% were correct at the genus level and 75.6% were correct at the species level at 24 h. In the total study of 352 strains, 46.6% were correct to the species level at 4 h and 57.1% were correct to the species level at 24 h. The error rate was 10.4% after 4 h and 9.6% after 24 h. The Biolog performed well with many genera, but problems were encountered with some strains of Klebsiella, Enterobacter, and Serratia. We found the system to be versatile and easy to use.
机译：Biolog鉴定系统（Biolog，Inc.，Hayward，CA）是一种新的细菌鉴定方法，可基于呼吸过程中产生的电子交换建立鉴定，从而导致随后的基于四氮唑的颜色变化。该系统测试微生物氧化一组95种不同碳源的能力。我们报告了该仪器使用其计算机驱动的酶免疫分析仪识别多种不同临床相关成员肠杆菌科和革兰氏阴性非肠杆菌科的能力的初步调查。 Biolog报告了对352种受测生物中的266种的鉴定（正确或不正确）（75.6％）。在报告的266种鉴定中，在24 h时属的正确率为87.3％，在物种中的正确率为75.6％。在对352个菌株的总体研究中，46.6％的物种水平在4 h正确，57.1％的物种水平在24 h正确。 4小时后错误率为10.4％，24小时后错误率为9.6％。 Biolog在许多属上表现良好，但是在某些克雷伯菌，肠杆菌和沙雷氏菌中遇到了问题。我们发现该系统通用且易于使用。

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