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首页> 外文期刊>Journal of Clinical Microbiology >Evaluation of a new system for the rapid identification of clinically important yeasts.
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Evaluation of a new system for the rapid identification of clinically important yeasts.

机译:快速鉴定临床上重要酵母的新系统的评估。

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The rapid system developed by Huppert et al. (1975) for the identification of yeasts based on assimilation and fermentation patterns and on germ tube and pseudohyphal production was evaluated in a comparative study with conventional procedures. The 95 test cultures were members of the genera Candida, Cryptococcus, Rhodotorula, Saccharomyces, Torulopsis, and Trichosporon. The study revealed that approximately 94% of the isolates were correctly identified by the rapid method in comparison with the standard method. With the rapid method identification was accomplished in 72h, and with the conventional procedures identification was completed in 2 weeks. Although it was difficult with some isolates to obtain definitive speciation by the rapid method, this method promises to be especially useful in clinical laboratories for the identification of yeasts of medical importance. Modifications were made in the procedure of Huppert et al. (1975) to improve the reading of reactions. Commercial media and a disk dispenser to make the method more useful were also investigated.
机译:Huppert等人开发的快速系统。 (1975)基于同化和发酵模式以及胚芽管和假菌丝生产的酵母鉴定在常规研究的比较研究中进行了评估。 95个测试培养物分别是假丝酵母,隐球菌,杜鹃花,酿酒酵母,Torulopsis和Trichosporon的成员。研究表明,与标准方法相比,快速方法可正确鉴定出约94%的分离物。快速方法鉴定在72小时内完成,常规方法鉴定在2周内完成。尽管某些分离物很难通过快速方法获得确定的物种,但该方法有望在临床实验室中特别有用,以鉴定具有医学重要性的酵母。在Huppert等人的方法中进行了修改。 (1975)提高反应的阅读。还研究了商业媒体和使该方法更有用的磁盘分配器。

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