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首页> 外文期刊>Journal of Clinical Microbiology >Immune electron microscopy of avian infectious bronchitis virus serotypes.
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Immune electron microscopy of avian infectious bronchitis virus serotypes.

机译：禽传染性支气管炎病毒血清型的免疫电子显微镜检查。

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An immune electron microscopy agglutination technique in which emphasis is placed upon the importance of antigen-antibody equivalence has been developed as a possible method for the serotyping of avian infectious bronchitis viruses. The Connecticut and Massachusetts 41 serotypes were used as a model system. Stock virus concentrations were standardized by physical particle counts of virions sedimented directly onto electron microscope specimen grids. Suspensions containing approximately 150 virions per grid square were allowed to react with dilutions of homologous and heterologous antisera. Virions in these constant virus-variable serum mixtures were sedimented directly onto electron microscope specimen grids, and the relative degree of aggregation per grid was determined from the mean percent aggregation of five randomly selected grid squares. In homologous assays, regions of relative antibody excess, of equivalence, and of relative antigen excess were clearly evident. At equivalence, the mean percent aggregation was significantly higher than in the regions of relative antibody or antigen excess. In the heterologous systems, the degree of aggregation differed little from that of the virus controls containing no antiserum.
机译：已经开发了一种免疫电子显微镜凝集技术，其着重于抗原-抗体等效性的重要性，作为禽传染性支气管炎病毒血清分型的一种可能方法。康涅狄格州和马萨诸塞州的41种血清型被用作模型系统。通过直接沉积在电子显微镜标本格栅上的病毒粒子的物理颗粒计数来标准化原病毒浓度。允许每个网格平方包含大约150个病毒粒子的悬浮液与稀释的同源和异源抗血清反应。将这些恒定病毒可变血清混合物中的病毒颗粒直接沉淀到电子显微镜标本网格上，并根据五个随机选择的网格正方形的平均聚集百分比确定每个网格的相对聚集度。在同源测定中，相对抗体过量，当量和抗原相对过量的区域是清楚可见的。当量相等时，平均聚集百分比显着高于相对抗体或抗原过量区域。在异源系统中，聚集程度与不含抗血清的病毒对照的聚集程度差异很小。

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