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首页> 外文期刊>Journal of Clinical Microbiology >Serological diagnosis of influenza B virus infection: comparison of an enzyme-linked immunosorbent assay and the hemagglutination inhibition test.
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Serological diagnosis of influenza B virus infection: comparison of an enzyme-linked immunosorbent assay and the hemagglutination inhibition test.

机译：乙型流感病毒感染的血清学诊断：酶联免疫吸附试验与血凝抑制试验的比较。

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摘要

The sensitivity of an enzyme-linked immunosorbent assay (ELISA) for the detection of antibody to influenza B virus was compared with that of the hemagglutination inhibition test on acute- and convalescence-phase sera obtained from adults and children infected with influenza B virus. Two whole virus, tissue culture-grown antigen preparations were used in the ELISA, influenza B/West Virginia/81 and influenza B/Hong Kong/72. Four antigens were used in the hemagglutination inhibition test. These included the tissue culture-grown whole virus antigens that were used in the ELISA. In addition the standard egg-grown antigens, influenza B/Singapore/79 and influenza B/Hong Kong/72, were included for comparison. The ELISA antibody titer was significantly correlated to the hemagglutination inhibition antibody titer, and 10 of 10 adults and 17 of 21 children infected with influenza B had fourfold antibody increases as detected by ELISA with either antigenic type of tissue culture-grown whole virus. Increases in geometric mean antibody titers of 16- to 71-fold were detected by ELISA. Increases in geometric mean antibody titers of 3- to 10-fold were detected by hemagglutination inhibition depending on the type of antigen utilized. We found that ELISA with whole virus antigens was a sensitive and specific test for the detection of antibody to influenza B virus.
机译：将酶联免疫吸附测定（ELISA）检测针对B型流感病毒的抗体的敏感性与针对从感染B型流感病毒的成人和儿童获得的急性期和恢复期血清的血凝抑制试验的敏感性进行了比较。 ELISA中使用了两种全病毒，组织培养生长的抗原制剂，分别为B型流感/西弗吉尼亚州/ 81和B型流感/香港/ 72。血凝抑制试验中使用了四种抗原。这些包括用于ELISA的组织培养生长的全病毒抗原。另外，还包括了标准的卵生抗原，即B型流感/新加坡/ 79和B型流感/香港/ 72。 ELISA抗体滴度与血细胞凝集抑制抗体滴度显着相关，用两种抗原类型的组织培养生长全病毒通过ELISA检测，感染B型流感的10名成人中的10名和21名儿童中的17名具有四倍的抗体增加。通过ELISA检测到几何平均抗体滴度增加了16至71倍。通过血凝抑制作用检测到的几何平均抗体滴度增加了3至10倍，具体取决于所用抗原的类型。我们发现，使用完整病毒抗原的ELISA是检测B型流感病毒抗体的灵敏且特异的测试。

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