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首页> 外文期刊>Journal of Clinical Microbiology >Detection of group B streptococcal antigens in amniotic fluid of rhesus monkeys.
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Detection of group B streptococcal antigens in amniotic fluid of rhesus monkeys.

机译：恒河猴羊水中B组链球菌抗原的检测。

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To simulate group B streptococci (GBS) amniotic fluid infections common in humans and to examine bacterial growth and the appearance of GBS antigens in vivo, GBS were injected into the amniotic cavity of 19 near-term rhesus monkeys. Transabdominal aspirates of amniotic fluid were obtained before bacterial challenge, after 2 and 6 h, and during cesarean section delivery (24 h). Each fluid was quantitatively cultured for GBS. Specimens of amniotic fluid and gastric aspirate from each infant were tested for the presence of GBS antigens with a commercial latex particle agglutination test (Wellcogen Strep B; Wellcome Diagnostics, Dartford, England). To eliminate nonspecific latex particle agglutination reactivity, presumably caused by proteins, a processing procedure was required. Despite active proliferation of bacteria, only 12% of the 2-h amniotic specimens were latex particle agglutination positive. In contrast, 94% of th3 6-h and 100% of the 24-h specimens had detectable antigens, as did 89% of the gastric fluid specimens aspirated from the 19 newborns. Latex particle agglutination tests, after proper processing, will readily detect GBS antigens in amniotic or gastric aspirate fluid from experimentally infected rhesus monkeys.
机译：为了模拟人类常见的B组链球菌（GBS）羊水感染并检查体内细菌的生长和GBS抗原的出现，将GBS注入19只近期恒河猴的羊膜腔中。在细菌攻击前，2和6小时后以及剖宫产（24 h）期间，获取经腹腔吸出的羊水。对每种液体进行GBS定量培养。用商业乳胶颗粒凝集试验（Wellcogen Strep B; Wellcome Diagnostics，Dartford，England），测试每个婴儿的羊水和胃抽吸物的标本中是否存在GBS抗原。为了消除可能由蛋白质引起的非特异性胶乳颗粒凝集反应性，需要加工程序。尽管细菌活跃增殖，但2-h羊膜标本中只有12％的乳胶颗粒凝集呈阳性。相比之下，th3 6-h标本中的94％和24-h标本中的100％具有可检测到的抗原，从19个新生儿中吸出的胃液标本中有89％也是如此。乳胶颗粒凝集试验经过适当处理后，可以很容易地检测出实验感染的恒河猴的羊水或胃液中的GBS抗原。

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