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首页> 外文期刊>Journal of Clinical Microbiology >Semisolid blood-free selective-motility medium for the isolation of campylobacters from stool specimens.
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Semisolid blood-free selective-motility medium for the isolation of campylobacters from stool specimens.

机译：半固体无血选择性运动培养基，用于从粪便标本中分离弯曲杆菌。

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Isolation of Campylobacter jejuni and C. coli from stool specimens is done by growing campylobacter colonies on solid selective media with or without blood. However, recognition of these colonies can be difficult. Therefore, we decided to evaluate an isolation procedure based on the swarming of campylobacters through a semisolid medium. We developed a semisolid blood-free selective motility (SSM) medium which is composed of Mueller-Hinton broth with 0.4% agar and supplemented with cefoperazone (30 micrograms/ml) and trimethoprim (50 micrograms/ml). The SSM medium was compared with our previously described Butzler Medium Virion (Goossens et al., J. Clin. Microbiol. 24:840-843, 1986) and blood-free medium (Bolton and Coates, J. Appl. Bacteriol. 54:115-125, 1983) with cefoperazone (32 micrograms/ml) (Bolton et al., J. Clin. Pathol. 37:956-957, 1986). Of 1,890 routine stool specimens tested, 100 were found to be positive for campylobacters: 95 were recovered with the SSM medium, 94 with the Virion medium, and 90 with the blood-free medium. The SSM medium performed equally well whether it was incubated in the special incubator or the candle jar. Only 4.4 and 7.3% of the plates grew contaminating fecal flora when incubated in the special incubator and the candle jar, respectively. Clearly the SSM medium is easy, quick, cheap, sensitive, and more selective than any other medium which has been developed so far and does not require the addition of blood. We believe that this medium has a future in the routine microbiology laboratory in developed as well as in developing countries.
机译：从粪便标本中分离空肠弯曲杆菌和大肠杆菌是通过在有或没有血液的固体选择性培养基上生长弯曲杆菌菌落来完成的。但是，很难识别这些菌落。因此，我们决定基于通过半固体培养基中弯曲杆菌群的分离方法进行评估。我们开发了一种半固态无血选择性运动（SSM）培养基，该培养基由含0.4％琼脂的Mueller-Hinton肉汤组成，并补充有头孢哌酮（30微克/毫升）和甲氧苄啶（50微克/毫升）。将SSM培养基与我们先前描述的Butzler培养基病毒体（Goossens等，J. Clin。Microbiol.24：840-843，1986）和无血培养基（Bolton and Coates，J. Appl。Bacteriol。54： 115-125，1983）与头孢哌酮（32微克/毫升）（Bolton等人，J。Clin。Pathol。37：956-957，1986）。在测试的1,890个常规粪便标本中，发现100株弯曲杆菌呈阳性：用SSM培养基回收了95份，使用Virion培养基回收了94份，使用无血培养基回收了90份。无论是在特殊的培养箱中还是在烛罐中进行培养，SSM培养基的性能均相同。当分别在特殊的培养箱和蜡烛罐中培养时，分别只有4.4％和7.3％的板污染了粪便菌群。显然，SSM培养基比迄今已开发的不需要添加血液的任何其他培养基更容易，快速，便宜，灵敏且更具选择性。我们相信，这种培养基在发达国家和发展中国家的常规微生物实验室中都有着发展的前景。

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