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首页> 外文期刊>Journal of Clinical Microbiology >Comparison of a serum replacement (Omni Serum) and fetal bovine serum in cell cultures used to isolate herpes simplex virus from clinical specimens.
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Comparison of a serum replacement (Omni Serum) and fetal bovine serum in cell cultures used to isolate herpes simplex virus from clinical specimens.

机译：比较用于从临床标本中分离单纯疱疹病毒的细胞培养物中的血清替代品（Omni血清）和胎牛血清。

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Traditionally, fetal bovine serum (FBS) has been the principal component in media used in the growth and maintenance of cell cultures. Recent shortages have affected the cost and availability of FBS to clinical laboratories. Furthermore, lot-to-lot variability can affect cell culture performance and growth. We evaluated a commercially available serum replacement (Omni Serum; Advanced Biotechnologies Inc., Columbia, Md.) for use in the growth of cell cultures and for use in maintenance media used for the isolation of herpes simplex virus from clinical specimens. Cells (rhabdomyosarcoma and mink lung) raised on 5% Omni Serum grew as well as those grown on 10% FBS. The sensitivity of the Omni-raised cells to herpes simplex virus that had been isolated from 111 clinical specimens was equal to that of the cells raised and maintained with FBS. Cells grown with 10% FBS and maintained with 2% Omni Serum displayed the same sensitivity and integrity in tubes (rhabdomyosarcoma and mink lung) and vials (MRC-5 cells) as cells grown with 10% FBS and maintained with 5% FBS. This study indicates that Omni Serum is an acceptable substitute for FBS in maintenance media for cell culture tubes and vials used for viral isolation from clinical specimens.
机译：传统上，胎牛血清（FBS）已成为用于细胞培养物生长和维持的培养基中的主要成分。最近的短缺影响了FBS在临床实验室的成本和可用性。此外，批次间的差异会影响细胞培养的性能和生长。我们评估了市售的血清替代品（Omni Serum; Advanced Biotechnologies Inc.，哥伦比亚，马里兰州），用于细胞培养的生长以及用于从临床标本中分离单纯疱疹病毒的维持培养基。在5％Omni Serum上生长的细胞（横纹肌肉瘤和水貂肺）与在10％FBS上生长的细胞一样生长。从111个临床标本中分离出的全培养细胞对单纯疱疹病毒的敏感性与FBS培养和维持的细胞的敏感性相同。用10％FBS生长并维持2％Omni Serum的细胞在管（横纹肌肉瘤和水貂肺）和小瓶（MRC-5细胞）中显示出与使用10％FBS和维持5％FBS相同的敏感性和完整性。这项研究表明，在用于从临床标本中进行病毒分离的细胞培养管和小瓶的维护培养基中，全脂血清可以替代FBS。

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