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首页> 外文期刊>Journal of Clinical Microbiology >Hemolysins and other characteristics that help differentiate and biotype Staphylococcus lugdunensis and Staphylococcus schleiferi.
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Hemolysins and other characteristics that help differentiate and biotype Staphylococcus lugdunensis and Staphylococcus schleiferi.

机译：溶血素和其他有助于区分卢旺达氏葡萄球菌和schleiferi葡萄球菌并对其进行生物分类的特征。

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Reference strains and clinical isolates representing the newly defined species Staphylococcus lugdunensis and Staphylococcus schleiferi were examined with the battery of tests previously recommended (G.A. Hébert, C.G. Crowder, G.A. Hancock, W.R. Jarvis, and C. Thornsberry, J. Clin. Microbiol. 26:1939-1949, 1988) for other species of coagulase-negative staphylococci (CNS). The Staph-Ident system (Analytab Products, Plainview, N.Y.) supplemented with tests for synergistic hemolysis, adherence to glass, pyroglutamyl-beta-naphthylamide hydrolysis, and susceptibility to a set of five antimicrobial disks differentiated each of these species from other species of CNS and separated strains within each species into several biotypes. Most strains (95%) of S. lugdunensis produced a delta hemolysin like that seen with nine other species of CNS. Most strains (91%) of S. schleiferi produced a beta hemolysin, which is a unique characteristic among CNS. Most (95%) of the S. schleiferi but very few (12%) of the S. lugdunensis were adherence positive. Both hemolysins and adherins are potential virulence factors among CNS. Some (29%) of the S. lugdunensis were beta-lactamase positive. The S. lugdunensis were resistant to polymyxin B and bacitracin (10 U), but the S. schleiferi were susceptible to both disks. Clinical isolates of S. lugdunensis were aligned in 18 biotypes because of eight biochemical profiles and eight physiologic subtypes; isolates of S. schleiferi were in 8 biotypes because of three biochemical profiles and subtypes. These tools for correctly identifying and then biotyping two more clinical species of CNS should enhance both epidemiologic and ecologic investigations.
机译：用先前推荐的一系列检测方法（GAHébert，CG Crowder，GA Hancock，WR Jarvis和C.Thornsberry，J.Clin.Microbiol.26： （1939-1949，1988）用于其他物种的凝固酶阴性葡萄球菌（CNS）。 Staph-Ident系统（Analytab Products，Plainview，NY）补充了协同溶血，粘附于玻璃，焦谷氨酰-β-萘酰胺水解以及对一组五个抗菌盘的敏感性的测试，从而将这些物种与其他CNS物种区分开来并将每个物种内的菌株分为几种生物型。大部分lugdunensis菌株（95％）产生的δ溶血素与其他9种CNS一样。 schleiferi的大多数菌株（91％）产生β溶血素，这是中枢神经系统的独特特征。 schleiferi的大部分（95％），但lugdunensis的极少数（12％）的粘附阳性。溶血素和粘附素都是中枢神经系统中潜在的致病因子。卢登敦链球菌中有一些（29％）是β-内酰胺酶阳性的。卢格氏链球菌对多粘菌素B和杆菌肽（10 U）具有抗性，但schleiferi对两个圆盘均敏感。由于八种生化特征和八种生理亚型，沙门氏菌临床分离株分为18种生物型。由于三个生化特征和亚型，S。schleiferi的分离物有8种生物型。这些用于正确识别然后再对两种中枢神经系统临床物种进行生物分型的工具应能加强流行病学和生态学研究。

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