Use of automated sequencing of polymerase chain reaction-generated amplicons to identify three types of cholera toxin subunit B in Vibrio cholerae O1 strains.

O Olsvik; J Wahlberg; B Petterson; M Uhlén; T Popovic; I K Wachsmuth; P I Fields

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Use of automated sequencing of polymerase chain reaction-generated amplicons to identify three types of cholera toxin subunit B in Vibrio cholerae O1 strains.

机译：使用聚合酶链反应产生的扩增子的自动测序来鉴定霍乱弧菌O1菌株中的三种霍乱毒素亚基B。

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Cholera toxin is the principal factor causing the profuse intestinal fluid secretion that is characteristic of cholera. The DNA sequences of the cholera toxin subunit B structural genes from 45 Vibrio cholerae O1 strains isolated in 29 countries over a period of 70 years were determined by automated DNA sequencing of polymerase chain reaction-generated amplicons. Three types of cholera toxin B subunit gene (ctxB) were identified. Genotype 1 was found in strains of classical biotype worldwide and El Tor biotype strains associated with the U.S. Gulf Coast, genotype 2 was found in El Tor biotype strains from Australia, and genotype 3 was found in El Tor biotype strains from the seventh pandemic and the recent Latin American epidemic. All base changes correspond to an amino acid substitution in the B subunit of the cholera toxin. Heterogenicity in the B subunit could have implications for vaccine development and diagnostic tests for cholera toxin and antitoxin. We conclude that this technology provides timely and potentially useful epidemiological information.

机译：霍乱毒素是引起霍乱特征的大量肠道液体分泌的主要因素。通过对聚合酶链反应产生的扩增子进行自动DNA测序，确定了29个国家在70年内分离的45种霍乱弧菌O1菌株的霍乱毒素亚基B结构基因的DNA序列。确定了三种类型的霍乱毒素B亚基基因（ctxB）。在全球经典生物型菌株和与美国墨西哥湾沿岸相关的El Tor生物型菌株中发现了基因型1，在澳大利亚的El Tor生物型菌株中发现了基因型2，在第七次大流行和第二次大流行期间在El Tor生物型菌株中发现了基因型3。最近的拉丁美洲流行病。所有碱基变化都对应于霍乱毒素B亚基中的氨基酸取代。 B亚基的异源性可能对霍乱毒素和抗毒素的疫苗开发和诊断测试产生影响。我们得出的结论是，该技术提供了及时且可能有用的流行病学信息。

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《Journal of Clinical Microbiology》 |1993年第1期|共4页
作者
O Olsvik; J Wahlberg; B Petterson; M Uhlén; T Popovic; I K Wachsmuth; P I Fields;
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中图分类医学微生物学（病原细菌学、病原微生物学）;
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1. Use of automated sequencing of polymerase chain reaction-generated amplicons to identify three types of cholera toxin subunit B in Vibrio cholerae O1 strains. [J] . O Olsvik, J Wahlberg, B Petterson, Journal of Clinical Microbiology . 1993,第1期

机译：使用聚合酶链反应产生的扩增子的自动测序来鉴定霍乱弧菌O1菌株中的三种霍乱毒素亚基B。
2. RAPID DETECTION OF CHOLERA TOXIN GENE OF VIBRIO CHOLERAE O1 BY POLYMERASE CHAIN REACTION [J] . Ren Shaotang, Qin Yizhong, Zhang Xiaoqin, Chinese Medical Journal . 1996,第6期

机译：聚合酶链反应快速检测霍乱弧菌O1的霍乱毒素基因
3. RAPID DETECTION OF CHOLERA TOXIN GENE OF VIBRIO CHOLERAE O1 BY POLYMERASE CHAIN REACTION [J] . 任少堂, 秦一中, 张晓琴, 中华医学杂志：英文版 . 1996,第006期

机译：聚合酶链反应快速检测霍乱霍乱O1的霍乱毒素基因
4. Automated validation of polymerase chain reactions using amplicon melting curves [C] . Mann, T.P., Humbert, . 2005

机译：使用扩增子熔解曲线自动验证聚合酶链反应
5. Association of Cholera Toxin with Vibrio cholerae Outer Membrane Vesicles [D] . Rasti Boroujeni, Elnaz . 2020

机译：霍乱毒素与弧菌霍乱外膜囊泡的关联
6. Use of automated sequencing of polymerase chain reaction-generated amplicons to identify three types of cholera toxin subunit B in Vibrio cholerae O1 strains. [O] . O Olsvik, J Wahlberg, B Petterson, 1993

机译：使用聚合酶链反应产生的扩增子的自动测序来鉴定霍乱弧菌O1菌株中的三种霍乱毒素亚基B。
7. Use of automated sequencing of polymerase chain reaction-generated amplicons to identify three types of cholera toxin subunit B in Vibrio cholerae O1 strains [O] . O Olsvik, J Wahlberg, B Petterson, 1993

机译：使用聚合酶链反应产生的扩增子的自动测序以鉴定三种类型的霍乱霍乱O1菌株中的霍乱毒素亚基B.
8. Isolation and Characterization of a Vibrio cholerae Strain Unable to Secrete Cholera Toxin, and Cloning and Characterization of Genes from Vibrio cholerae that Confer a Congo Red Dye Binding Phenotype on Escherichia coli. [R] . Coker, C. 1996

机译：无法分泌霍乱毒素的霍乱弧菌菌株的分离和鉴定，以及霍乱弧菌基因的克隆和表征，该基因在大肠杆菌中具有刚果红染料结合表型。

Use of automated sequencing of polymerase chain reaction-generated amplicons to identify three types of cholera toxin subunit B in Vibrio cholerae O1 strains.

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