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首页> 外文期刊>Journal of Clinical Microbiology >Molecular typing of Pseudomonas pseudomallei: restriction fragment length polymorphisms of rRNA genes.
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Molecular typing of Pseudomonas pseudomallei: restriction fragment length polymorphisms of rRNA genes.

机译：Pseudomonas pseudomallei的分子分型：rRNA基因的限制性片段长度多态性。

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The aim of this study was to develop a typing scheme for Pseudomonas pseudomallei by comparison of patterns of restriction fragment length polymorphisms in rRNA genes (ribotyping). BamHI restriction digests of 100 isolates from various animal (34), human (58), and environmental (6) sources, including six reference strains, were hybridized to Escherichia coli 16S and 23S rRNAs. A chemiluminescent labelling and detection system was used to visualize bands. On the basis of patterns, the strains were classified into 22 different groups, with the largest containing 29 isolates. While most of the ribotypes were not exclusive to a particular source, some ribotypes were restricted to a particular geographic area or to either a human or a particular animal species. Application of the typing scheme to isolates of four independent outbreaks among animals showed that certain ribotypes predominated. The study demonstrated ribotyping to be a useful tool in epidemiological investigations of melioidosis.
机译：这项研究的目的是通过比较rRNA基因中限制性片段长度多态性的模式（核糖体分型），为假单胞菌假单胞菌开发一种分型方案。将100种来自多种动物（34），人（58）和环境（6）的分离株的BamHI限制性酶切消化物（包括6个参考菌株）与大肠杆菌16S和23S rRNA杂交。化学发光标记和检测系统用于可视化条带。根据模式，将菌株分为22个不同的组，最大的菌株包含29个分离株。尽管大多数核糖型并非特定来源所独有，但某些核糖型仅限于特定地理区域或人类或特定动物物种。将分型方案应用于动物中四个独立爆发的分离株表明，某些核糖型占主导地位。这项研究表明，核糖体分型是在类鼻li病流行病学调查中有用的工具。

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