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首页> 外文期刊>Journal of Clinical Microbiology >Development and evaluation of a blood-free medium for determining growth curves and optimizing growth of Rochalimaea henselae.
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Development and evaluation of a blood-free medium for determining growth curves and optimizing growth of Rochalimaea henselae.

机译:开发和评估无血培养基,以确定生长曲线并优化罗汉木的生长。

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摘要

Two strains of Rochalimaea henselae were used to optimize a blood-free growth medium. Seven agar bases, four broths, and combinations of eight supplements were evaluated. Acceptable growth was achieved in media containing Fildes solution and hemin, with the best growth demonstrated in brucella broth or on brucella agar with 6 to 8% Fildes solution and 250 micrograms of hemin per ml. R. henselae utilized hemin in concentrations six times that utilized by Rochalimaea quintana. Erythrocyte membrane was necessary to achieve the full growth-promoting effect of rabbit blood.
机译:两种罗汉木(Rochalimaea henselae)菌株用于优化无血生长培养基。评估了七个琼脂基质,四个肉汤以及八种补品的组合。在含有Fildes溶液和血红素的培养基中可以实现可接受的生长,其中以6%至8%的Fildes溶液和每毫升250微克的Hemin的布鲁氏肉汤或布鲁氏琼脂培养基表现出最佳生长。亨塞勒氏菌使用的血红素浓度是罗汉木(Rochalimaea quintana)的六倍。红血球膜是实现兔血充分生长促进作用所必需的。

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