Direct identification and typing of Mycobacterium tuberculosis by PCR.

H Neimark; M Ali Baig; S Carleton

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Direct identification and typing of Mycobacterium tuberculosis by PCR.

机译：通过PCR直接鉴定和鉴定结核分枝杆菌。

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We have developed a rapid PCR assay that types strains of Mycobacterium tuberculosis by generating distinct DNA fingerprints directly from primary cultures. This assay allows strain identification analogous to that achieved by the standard restriction fragment length polymorphism method, and fingerprints are obtained in less than 8 h. This assay does not require subculturing, DNA purification, restriction digestion, Southern blotting, or nucleic acid hybridization. Rapid and precise identification of M. tuberculosis strains permits immediate molecular epidemiologic studies. The assay can be converted to a computer-automated system by employing fluorescently labeled PCR primers and the Perkin-Elmer DNA sequencer so that unknown-specimen fingerprints are identified by computer comparison to a database of M. tuberculosis strain fingerprints.

机译：我们已经开发了一种快速PCR检测方法，可通过直接从原代培养物中产生独特的DNA指纹来对结核分枝杆菌菌株进行分型。该测定法允许菌株鉴定类似于通过标准限制性片段长度多态性方法获得的菌株鉴定，并且在少于8小时内获得指纹。该测定不需要传代，DNA纯化，限制性酶切，Southern印迹或核酸杂交。快速准确地鉴定结核分枝杆菌菌株可以立即进行分子流行病学研究。通过使用荧光标记的PCR引物和Perkin-Elmer DNA测序仪，可以将该测定法转换为计算机自动化系统，以便通过与结核分枝杆菌菌株指纹数据库进行计算机比较来鉴定未知样品的指纹。

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《Journal of Clinical Microbiology》 |1996年第10期|共6页
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H Neimark; M Ali Baig; S Carleton;
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中图分类医学微生物学（病原细菌学、病原微生物学）;
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1. Detection and identification of Mycobacterium tuberculosis directly from sputum sediments by Amplicor PCR. [J] . D F Moore, J I Curry Journal of Clinical Microbiology . 1995,第10期

机译：通过Amplicor PCR直接从痰液沉积物中检测和鉴定结核分枝杆菌。
2. Development of a novel DNA extraction method for identification and quantification of Mycobacterium avium subsp. paratuberculosis from tissue samples by real-time PCR. [J] . Park K. T., Allen A. J., Davis W. C. Journal of Microbiological Methods . 2014,第Null期

机译：鉴定和定量鸟分枝杆菌亚种的新型DNA提取方法的开发。通过实时PCR从组织样本中检出副结核病。
3. Direct detection of Mycobacterium avium subsp. paratuberculosis in bovine milk by multiplex Real-time PCR. [J] . Selim A., El-Haig M., Galila E., Animal Science Papers and Reports . 2013,第4期

机译：直接检测鸟分枝杆菌亚种。实时荧光定量PCR检测牛乳中的副结核病。
4. Identification of Mycobacterium avium subsp. paratuberculosis in tissue and faecal material of sheep by PCR. Comparison with serological data [C] . De Grossi L, Di Donato F, Micarelli G, International Colloquium on Paratuberculosis . 2007

机译：分枝杆菌亚空间的鉴定。 PCR绵羊组织和粪便材料的抗麻痹性。与血清数据进行比较
5. Type I Interferon Induction by Diverse Strains of the Mycobacterium Tuberculosis Complex. [D] . Wiens, Kirsten E. 2017

机译：不同菌株结核分枝杆菌复合物的I型干扰素诱导。
6. Direct identification and typing of Mycobacterium tuberculosis by PCR. [O] . H Neimark, M Ali Baig, S Carleton 1996

机译：通过PCR直接鉴定和分型结核分枝杆菌。
7. Direct identification and typing of Mycobacterium tuberculosis by PCR [O] . H Neimark, M Ali Baig, S Carleton 1996

机译：通过PCR直接鉴定和键入结核分枝杆菌

Direct identification and typing of Mycobacterium tuberculosis by PCR.

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