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首页> 外文期刊>Journal of Clinical Microbiology >Complement-fixing immunoglobulin M antibody response in patients with infantile gastroenteritis.
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Complement-fixing immunoglobulin M antibody response in patients with infantile gastroenteritis.

机译：婴幼儿胃肠炎患者中补体结合免疫球蛋白M抗体反应。

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摘要

Complement-fixing immunoglobulin M antibody to infantile gastroenteritis virus (a rotavirus) was detected with highest sensitivity when the antibody-antigen-complement mixture was incubated at 37 degrees C for 1 h prior to the addition of sensitized sheep erythrocytes. Sucrose gradient centrifugation of sera collected sequentially from four patients after infection detected 19S complement-fixing antibody up to 5 weeks, with highest titers at 1 week, after the onset of illness. Treatment of the whole sera with 2-mercaptoethanol decreased complement-fixing titers only up to 2 weeks after onset of illness.
机译：在添加致敏的绵羊红细胞之前，将抗体-抗原-补体混合物在37°C下孵育1小时，从而以最高的灵敏度检测到针对婴儿肠胃炎病毒（轮状病毒）的补体固定免疫球蛋白M抗体。感染后从四名患者中依次收集的蔗糖梯度离心血清检测到发病后长达5周的19S补体结合​​抗体，滴度最高于1周。用2-巯基乙醇治疗整个血清仅在发病后2周内降低补体固定效价。

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