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首页> 外文期刊>Journal of Clinical Microbiology >Comparison of rates of virus isolation from leukocyte populations separated from blood by conventional and Ficoll-Paque/Macrodex methods.
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Comparison of rates of virus isolation from leukocyte populations separated from blood by conventional and Ficoll-Paque/Macrodex methods.

机译：通过常规方法和Ficoll-Paque / Macrodex方法从血液分离的白细胞群体中分离病毒的速率比较。

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One hundred fifty-two blood specimens, largely from immunocompromised patients, were collected in heparinized Vacutainer tubes and divided into paired aliquots of equal volume. Buffy-coat preparations, containing mixed leukocyte and separate mononuclear and polymorphonuclear leukocyte populations were obtained by treatment of blood with conventional and Ficoll-Paque/Macrodex (F-P/M) methods. The development of cytopathic effect in monolayers of WI-38 fibroblasts inoculated with cell suspensions derived from the two methods was used to assess virus infectivity. Twice as many virus isolations were obtained using F-P/M. Of those viruses isolated by both conventional and F-P/M, the development of cytopathic effect was more extensive using the latter method. Moreover, a greater variety of viruses was isolated using F-P/M method, as compared to the conventional method. The F-P/M method is no more time consuming than conventional procedures, is readily adaptable for use in the diagnostic virology laboratory, requires only minimal additional cost, and is a particularly suitable and effective means of monitoring viremia.
机译：在肝素化的Vacutainer管中收集了152个主要来自免疫功能低下患者的血液样本，并分成等体积的成对等份试样。通过用常规方法和Ficoll-Paque / Macrodex（F-P / M）方法处理血液，可获得包含混合白细胞以及单独的单核和多形核白细胞群的血沉棕黄层制剂。用两种方法衍生的细胞悬液接种的WI-38成纤维细胞单层细胞病变效应的发展用于评估病毒的感染性。使用F-P / M获得的病毒分离率是两倍。在通过常规方法和F-P / M方法分离出的那些病毒中，使用后一种方法可产生更广泛的细胞病变作用。此外，与常规方法相比，使用F-P / M方法分离了更多种病毒。 F-P / M方法不比常规程序耗时，容易适应在诊断病毒学实验室中使用，仅需极少的额外费用，是一种特别合适且有效的监测病毒血症的方法。

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