...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Clinical Microbiology >Sensitivity of immunofluorescence with monoclonal antibodies for detection of Chlamydia trachomatis inclusions in cell culture.
【24h】

Sensitivity of immunofluorescence with monoclonal antibodies for detection of Chlamydia trachomatis inclusions in cell culture.

机译：单克隆抗体对免疫荧光检测细胞培养中沙眼衣原体包涵体的敏感性。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Monoclonal antibodies which recognize the species-specific major outer membrane protein antigen of Chlamydia trachomatis were used for immunofluorescence staining of chlamydial inclusions in cell culture. A total of 115 clinical specimens were inoculated onto replicate HeLa 229 cell monolayers and assayed for chlamydial inclusions by immunofluorescence staining and Giemsa staining. Of the isolates, 38 were detected by immunofluorescence staining on passage 1 and 1 was detected on passage 2; 23 isolates on passage 1 and 13 isolates on passage 2 were detected by Giemsa staining. Immunofluorescence staining was significantly more sensitive than Giemsa staining for detecting chlamydial inclusions, particularly from specimens containing low titers of Chlamydia.
机译：识别沙眼衣原体物种特异性主要外膜蛋白抗原的单克隆抗体用于细胞培养中衣原体包涵体的免疫荧光染色。将总共​​115个临床标本接种到复制的HeLa 229细胞单层上，并通过免疫荧光染色和Giemsa染色检测衣原体包涵体。在分离株中，通过免疫荧光染色在第1代中检测到38个，在第2代中检测到1个;通过吉姆萨染色检测到第1代的23个分离株和第2代的13个分离株。免疫荧光染色比Giemsa染色对检测衣原体包涵体的敏感性要高得多，特别是对于含有低滴度衣原体的标本而言。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号