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首页> 外文期刊>Journal of Clinical Microbiology >Enzyme-linked immunosorbent assay for identification of infectious pancreatic necrosis virus.
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Enzyme-linked immunosorbent assay for identification of infectious pancreatic necrosis virus.

机译:酶联免疫吸附法鉴定传染性胰腺坏死病毒。

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摘要

The sensitivity, reproducibility, and specificity of an enzyme-linked immunosorbent assay for the identification of infectious pancreatic necrosis virus was determined. It was shown that 10(5.5) tissue culture infectious doses could be assayed by this method. The assay provided significant advantages in terms of time and ease of performance over the routinely employed serological tests. When an antiserum against a single isolate was used, it was possible to identify nine of ten North American isolates, four of six European isolates, and one isolate from Taiwan.
机译:确定了用于鉴定传染性胰腺坏死病毒的酶联免疫吸附测定的灵敏度,可重复性和特异性。结果表明,该方法可以测定10(5.5)个组织培养的感染剂量。与常规的血清学测试相比,该方法在时间和操作简便性方面均具有明显优势。当使用针对单个分离株的抗血清时,可以鉴定出十个北美分离株中的九个,六个欧洲分离株中的四个,以及来自台湾的一个分离株。

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