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首页> 外文期刊>Journal of Clinical Microbiology >Evaluation of the microenzyme-linked immunosorbent assay with Treponema pallidum antigen.
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Evaluation of the microenzyme-linked immunosorbent assay with Treponema pallidum antigen.

机译：梅毒螺旋体抗原与微酶联免疫吸附测定的评估。

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摘要

Whole-cell sonicates of Treponema pallidum, Nichols strain, were evaluated in an enzyme-linked immunosorbent assay (Elisa) for syphilis, and results were read in a Dynatek Microelisa Reader. The antigen was evaluated with sera from patients with syphilis, persons presumed normal, and biological false-positives. Two hundred and ninety-seven sera were tested by the ELISA with T. pallidum antigens, the Venereal Disease Research Laboratory (VDRL) slide test, the fluorescent treponemal antibody absorption (FTA-Abs) test, and the microhemagglutination assay for T. pallidum antibodies (MHA-TP). The results of all of the tests were compared. The ELISA, with 89.3% sensitivity, was less sensitive than the VDRL (93.3%) and FTA-Abs (100.0%) tests but more sensitive than the MHA-TP (76.0%). THe ELISA was considerably more sensitive in primary syphilis than the MHA-TP. Specificity was as follows: ELISA, 98.5%; FTA-Abs test, 97.8%; MHA-TP, 98.2%; and VDRL test, 92.7%. The ELISA has good potential as a confirmatory test in the serodiagnosis of syphilis.
机译：在梅毒梅毒的酶联免疫吸附测定（Elisa）中评估了梅毒螺旋体的全细胞超声处理，并在Dynatek Microelisa Reader中读取了结果。用梅毒患者，假定正常人和生物学假阳性患者的血清评估抗原。用梅毒螺旋体ELISA法检测了297份血清，性病研究实验室（VDRL）玻片试验，荧光螺旋体抗体吸收（FTA-Abs）试验和梅毒螺旋体微血凝试验。 （MHA-TP）。比较所有测试的结果。 ELISA的灵敏度为89.3％，比VDRL（93.3％）和FTA-Abs（100.0％）测试的灵敏度低，但比MHA-TP（76.0％）的灵敏度更高。 ELISA对原发性梅毒的敏感性比MHA-TP高得多。特异性如下：ELISA，98.5％; FTA-Abs测试，占97.8％; MHA-TP，98.2％;和VDRL测试占92.7％。 ELISA在梅毒的血清学诊断中具有作为确认试验的良好潜力。

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