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首页> 外文期刊>Journal of Clinical Microbiology >Analysis of the disagreement between automated bioluminescence-based and culture methods for detecting significant bacteriuria, with proposals for standardizing evaluations of bacteriuria detection methods.
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Analysis of the disagreement between automated bioluminescence-based and culture methods for detecting significant bacteriuria, with proposals for standardizing evaluations of bacteriuria detection methods.

机译：分析基于自动生物发光的方法与用于检测重要细菌尿的培养方法之间的分歧，并提出标准化评估细菌尿检测方法的建议。

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摘要

A fully automated method for detecting significant bacteriuria is described which uses firefly luciferin and luciferase to detect bacterial ATP in urine. The automated method was calibrated and evaluated, using 308 urine specimens, against two reference culture methods. We obtained a specificity of 0.79 and sensitivity of 0.75 using a quantitative pour plate reference test and a specificity of 0.79 and a sensitivity of 0.90 using a semiquantitative standard loop reference test. The majority of specimens negative by the automated test but positive by the pour plate reference test were specimens which grew several bacterial species. We suggest that such disagreement was most likely for urine containing around 10(5) colony-forming units per ml (the culture threshold of positivity) and that these specimens were ones contaminated by urethral or vaginal flora. We propose standard procedures for calibrating and evaluating rapid or automated methods for the detection of significant bacteriuria and have analyzed our results using these procedures. We recommend that identical analyses should be reported for other evaluations of bacteriuria detection methods.
机译：描述了一种检测重大细菌尿症的全自动方法，该方法使用萤火虫荧光素和荧光素酶来检测尿液中的细菌ATP。针对两种参考培养方法，使用308个尿液样本对自动化方法进行了校准和评估。使用定量倾倒板参考测试，我们获得了0.79的特异性和0.75的灵敏度;使用半定量标准环参考测试，我们获得了0.79的特异性和0.90的灵敏度。自动测试呈阴性但倾倒板参考测试呈阳性的大多数标本是生长了几种细菌的标本。我们建议这种异议最可能发生在每毫升尿液含有大约10（5）个菌落形成单位（阳性培养阈值）的情况下，并且这些标本是被尿道或阴道菌群污染的标本。我们提出了用于校准和评估检测重要细菌尿症的快速或自动化方法的标准程序，并使用这些程序分析了我们的结果。我们建议应报告相同的分析结果，以进行其他细菌尿检出方法的评估。

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