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首页> 外文期刊>Journal of Clinical Microbiology >Use of trehalose-mannitol-phosphatase agar to differentiate Staphylococcus epidermidis and Staphylococcus saprophyticus from other coagulase-negative staphylococci.
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Use of trehalose-mannitol-phosphatase agar to differentiate Staphylococcus epidermidis and Staphylococcus saprophyticus from other coagulase-negative staphylococci.

机译：使用海藻糖-甘露醇磷酸酶琼脂将表皮葡萄球菌和腐生葡萄球菌与其他凝固酶阴性葡萄球菌区分开。

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Using a plate medium containing trehalose, mannitol, and phenolphthalein diphosphate (TMPA), we differentiated significant clinical isolates of Staphylococcus epidermidis by their lack of acid production in 18 h from other coagulase-negative staphylococci, with our results having a sensitivity (R. S. Galen and S. R. Gambino, Beyond Normality: The Predictive Value and Efficiency of Medical Diagnoses) of 100%, a specificity of 89.9%, and a positive predictive value of 94.8%. With a Taxo A bacitracin disk, which differentiates Staphylococcus species from Micrococcus species, no zone of inhibition was seen for 96% of all staphylococcal strains, with 5 of 26 strains of Staphylococcus saprophyticus exhibiting zone diameters up to 10 mm. By using resistance to a 5-microgram novobiocin disk, we differentiated S. saprophyticus, with our results having a sensitivity of 100%, a specificity of 97.1%, and a positive predictive value of 83.9% on TMPA. These two species represented 77.8% of coagulase-negative staphylococci isolated. Reference strains fo Staphylococcus and Micrococcus species were differentiated by TMPA. The cost of TMPA was compared with that of another method. TMPA was found to offer an inexpensive, sensitive method for rapidly differentiating coagulase-negative Staphylococcus isolates.
机译：使用含有海藻糖，甘露醇和酚酞二磷酸（TMPA）的平板培养基，我们区分了表皮葡萄球菌的重要临床分离株，因为它们在18小时内没有产生酸，与其他凝固酶阴性葡萄球菌分离，我们的结果具有敏感性（RS Galen和SR Gambino，“超出正常值：医学诊断的预测价值和效率”为100％，特异性为89.9％，阳性预测值为94.8％。使用可区分葡萄球菌物种和微球菌物种的Taxo A杆菌肽盘，在所有葡萄球菌菌株中96％均未见抑菌圈，在26株腐生葡萄球菌菌株中有5株的抑菌圈直径高达10 mm。通过使用对5毫克新霉素圆盘的抗性，我们区分了腐生链球菌，我们的结果对TMPA的敏感性为100％，特异性为97.1％，阳性预测值为83.9％。这两个物种占分离的凝固酶阴性葡萄球菌的77.8％。用TMPA区分葡萄球菌和微球菌的参考菌株。将TMPA的成本与另一种方法的成本进行了比较。发现TMPA提供了一种廉价，灵敏的方法，用于快速区分凝固酶阴性葡萄球菌分离株。

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