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首页> 外文期刊>Journal of Clinical Microbiology >Enzyme-linked immunosorbent assay for detection of equine infectious anemia virus p26 antigen and antibody.
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Enzyme-linked immunosorbent assay for detection of equine infectious anemia virus p26 antigen and antibody.

机译:酶联免疫吸附法用于检测马传染性贫血病毒p26抗原和抗体。

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摘要

A sensitive specific enzyme-linked immunosorbent assay utilizing purified p26 antigen was developed for the detection of antibodies to equine infectious anemia virus in naturally and experimentally infected horses. Generally, antibodies to the virus could be detected by the enzyme-linked immunosorbent assay 3 to 4 days earlier than by the standard agar gel immunodiffusion test, and they could be detected more reliably in horses with weak or equivocal agar gel immunodiffusion test reactions. The enzyme-linked immunosorbent assay was also successfully applied to the detection of p26 antigen in tissue culture fluids.
机译:开发了利用纯化的p26抗原的灵敏的特异性酶联免疫吸附测定法,用于在自然和实验感染的马匹中检测感染性贫血病毒的抗体。通常,与标准琼脂凝胶免疫扩散试验相比,可通过酶联免疫吸附试验提前3至4天检测到该病毒的抗体,并且在琼脂凝胶免疫扩散试验较弱或含糊不清的马匹中,可以更可靠地检测到该抗体。酶联免疫吸附试验也成功地用于组织培养液中p26抗原的检测。

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