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首页> 外文期刊>Journal of Clinical Microbiology >Rapid diagnosis of cholera by coagglutination test using 4-h fecal enrichment cultures.
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Rapid diagnosis of cholera by coagglutination test using 4-h fecal enrichment cultures.

机译：使用4小时粪便富集培养物通过凝集试验快速诊断霍乱。

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A simple, rapid, and reliable method to detect Vibrio cholerae in fecal specimens would assist in the management of cases of severe diarrhea, especially since most such cases occur in areas with minimal laboratory facilities. A coagglutination test was used to detect V. cholerae antigen in bile-peptone broth incubated with feces. In the technique, Staphylococcus aureus Cowan 1 coated with anti-V. cholerae O1 antiserum was tested with cultures incubated for 4 h. When 165 specimens were tested, the sensitivity, specificity, and accuracy of the test, compared with standard culture methods, were 97, 99, and 98%, respectively. These promising results were better than those of dark-field microscopy using the same specimens, and the test was logistically easy to perform. The coagglutination test using enrichment broth culture of feces is a simple and rapid method which may be used to confirm a diagnosis of cholera.
机译：一种简单，快速且可靠的检测粪便标本中霍乱弧菌的方法将有助于处理严重腹泻的病例，特别是由于大多数此类病例发生在实验室设施最少的地区。用凝集试验检测与粪便一起培养的胆汁-蛋白p肉汤中的霍乱弧菌抗原。在该技术中，金黄色葡萄球菌Cowan 1涂有抗V。用培养4小时的培养物测试霍乱O1抗血清。当测试165个样本时，与标准培养方法相比，测试的敏感性，特异性和准确性分别为97％，99％和98％。这些有希望的结果比使用相同样本的暗视野显微镜要好，并且该测试从逻辑上讲易于执行。使用粪便富集培养液进行的凝集试验是一种简单而快速的方法，可用于确认霍乱的诊断。

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