...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Clinical Microbiology >Randomly amplified polymorphic DNA PCR for comparison of Mycobacterium abscessus strains from nosocomial outbreaks.
【24h】

Randomly amplified polymorphic DNA PCR for comparison of Mycobacterium abscessus strains from nosocomial outbreaks.

机译：随机扩增的多态性DNA PCR用于比较医院爆发的脓肿分枝杆菌菌株。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Mycobacterium abscessus is an important cause of water-related nosocomial outbreaks or pseudo-outbreaks. Strain comparison has relied on pulsed-field gel electrophoresis (PFGE). Unfortunately, almost 50% of strains cannot be assessed by this method. We studied 118 strains of M. abscessus previously studied by PFGE by randomly amplified polymorphic DNA (RAPD) PCR, including isolates from eight nosocomial outbreaks. Ten random primers were evaluated by using DNA prepared by boiling or phenol-chloroform extraction. Both DNA preparations gave the same grouping of isolates for three outbreaks compared to the groupings obtained by PFGE. Five outbreaks due to M. abscessus which gave broken DNA by PFGE gave evaluable patterns when studied by RAPD-PCR, with isolate clustering being consistent with that from other laboratory and epidemiologic data. The patterns were highly method dependent, strain comparison required the use of multiple primers, and the method worked best with purified DNA and by using strains for comparison on the same gel. We propose categories of indistinguishable, different, and inconclusive when comparing strains by RAPD-PCR. This study demonstrates that RAPD-PCR can be used for genetic comparison of M. abscessus strains, including strains which cannot be compared by PFGE, but the potential for misinterpretation is greater than that by PFGE.
机译：脓肿分枝杆菌是与水有关的医院内暴发或假性暴发的重要原因。应变比较依赖于脉冲场凝胶电泳（PFGE）。不幸的是，这种方法无法评估近50％的菌株。我们通过随机扩增多态性DNA（RAPD）PCR研究了先前通过PFGE研究的118株脓肿分支杆菌，包括来自八个医院爆发的分离株。使用沸腾提取或苯酚-氯仿提取制备的DNA评估十个随机引物。与通过PFGE获得的分组相比，两种DNA制剂在三个暴发中均给出了相同的分离株分组。通过脓胸支原体引起的五次暴发通过PFGE导致DNA断裂，当通过RAPD-PCR研究时，其暴发模式可评估，分离株的聚类与其他实验室和流行病学数据一致。图案高度依赖于方法，菌株比较需要使用多个引物，并且该方法最适合纯化的DNA并通过在同一凝胶上使用菌株进行比较。当通过RAPD-PCR比较菌株时，我们提出了无法区分，不同和不确定的类别。这项研究表明，RAPD-PCR可用于脓肿分支杆菌菌株的遗传比较，包括无法用PFGE比较的菌株，但误解的可能性大于PFGE。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号