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首页> 外文期刊>Journal of Clinical Microbiology >Detection of Helicobacter pylori in Stool Specimens by PCR and Antigen Enzyme Immunoassay
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Detection of Helicobacter pylori in Stool Specimens by PCR and Antigen Enzyme Immunoassay

机译：PCR和抗原酶免疫法检测粪便标本中的幽门螺杆菌

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A highly sensitive seminested PCR assay to detectHelicobacter pylori DNA in feces was developed. PCR with stool specimens and a novel antigen enzyme immunoassay (EIA) forH. pylori detection in feces were evaluated as diagnostic tools and in follow-up with samples from 63 infected and 37 noninfected persons. Infected individuals received eradication therapy followed by endoscopic follow-up 35 days after the start of treatment. At that time, a second stool specimen was obtained from 55 of these patients. Before eradication, the sensitivity of PCR was 93.7% and that of EIA 88.9%. Specificities were 100 and 94.6%, respectively. Of the 55 follow-up specimens, 41 originated from patients from whom H. pylori had been eradicated. Of these, 21 were still positive by PCR and 13 were positive by EIA, indicating that 1 month may be too short a period for follow-up evaluation of stool specimens by these tests.
机译：建立了一种检测粪便中幽门螺杆菌DNA的高灵敏度半巢式PCR检测方法。用粪便样本进行PCR和针对H的新型抗原酶免疫测定（EIA）。粪便中的幽门螺杆菌检测被评估为诊断工具，并跟踪了63名感染者和37名未感染者的样本。受感染的个体在治疗开始后的35天接受根除治疗，然后进行内镜随访。当时，从其中55名患者中获得了第二份粪便标本。根除前，PCR的敏感性为93.7％，EIA的敏感性为88.9％。特异性分别为100和94.6％。在55份随访标本中，有41份来自 H患者。幽门螺旋杆菌已被根除。其中21份经PCR仍为阳性，而13份经EIA呈阳性，表明1个月对于通过这些测试进行粪便标本的后续评估可能太短了。

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